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Team:Peking S/project/wire/matrix
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===Orthogonal activating matrix=== | ===Orthogonal activating matrix=== | ||
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| - | <center>[[File:Orthogonal Activating Matrix.jpg|600px]] | + | <center>[[File:Orthogonal Activating Matrix.jpg|600px]]</center> |
''Figure 1 A graphical representation of the activation associated with five receiving systems and their corresponding inducer.The concentration of salicylate is 10^-4 mol/L. LuxI product and RhlI product is the 10 fold dilution of the supernatant from a overnight culture bearing pT7 controlled luxI generator and ptet controlled rhlI generator, respectively. The concentration of 3-oxododecyl-homoserine lactone(3OC12HSL) is 10^-8 mol/L, and the CinI product is a 1:100 dilution of the overnight culture broth. '' | ''Figure 1 A graphical representation of the activation associated with five receiving systems and their corresponding inducer.The concentration of salicylate is 10^-4 mol/L. LuxI product and RhlI product is the 10 fold dilution of the supernatant from a overnight culture bearing pT7 controlled luxI generator and ptet controlled rhlI generator, respectively. The concentration of 3-oxododecyl-homoserine lactone(3OC12HSL) is 10^-8 mol/L, and the CinI product is a 1:100 dilution of the overnight culture broth. '' | ||
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| + | '''Table 1 Original Data of the activating matrix, showing absolute GFP fluorescence(a.u.)''' | ||
[[File:SR_Matrix_table1.png|600px]] | [[File:SR_Matrix_table1.png|600px]] | ||
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| + | '''Table 2 Data of the activating matrix, average fold compared with blank samples calculated''' | ||
[[File:SR_Matrix_table2.png|600px]] | [[File:SR_Matrix_table2.png|600px]] | ||
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| + | '''Table 3 Data of the activating matrix, activating fold normalized''' | ||
[[File:SR_Matrix_table3.png|600px]]</center> | [[File:SR_Matrix_table3.png|600px]]</center> | ||
Revision as of 23:39, 5 October 2011
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Chemical Wire Toolbox
Introduction|Harvesting ‘Chemical Wires’ From Nature|Synthesizing Quorum Sensing Inverters|Orthogonal Activating Matrix
Orthogonal activating matrix
Figure 1 A graphical representation of the activation associated with five receiving systems and their corresponding inducer.The concentration of salicylate is 10^-4 mol/L. LuxI product and RhlI product is the 10 fold dilution of the supernatant from a overnight culture bearing pT7 controlled luxI generator and ptet controlled rhlI generator, respectively. The concentration of 3-oxododecyl-homoserine lactone(3OC12HSL) is 10^-8 mol/L, and the CinI product is a 1:100 dilution of the overnight culture broth.
Table 1 Original Data of the activating matrix, showing absolute GFP fluorescence(a.u.)
Table 2 Data of the activating matrix, average fold compared with blank samples calculated
Table 3 Data of the activating matrix, activating fold normalized
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