Team:Tokyo Metropolitan/Project/Killing
From 2011.igem.org
(Difference between revisions)
Line 23: | Line 23: | ||
<tr><td><u><b><font size="3">Cell Lysis</font></b></u><br> | <tr><td><u><b><font size="3">Cell Lysis</font></b></u><br> | ||
- | <table><tr valign="top"><td width=" | + | <table><tr valign="top"><td width="500"> |
Bacteriophage have cell lysis cassette S,R,Rz called "Holin-Endolysin". S gene encodes "Holin" that forming hole on inner membrane of host cell.R gene encodes "Endolysin" degradates murein in intermediate membrane of host cell(in membrane of host cell,not harmful).Rz is also know for the enzyme attack outer membrane with R gene.<br> | Bacteriophage have cell lysis cassette S,R,Rz called "Holin-Endolysin". S gene encodes "Holin" that forming hole on inner membrane of host cell.R gene encodes "Endolysin" degradates murein in intermediate membrane of host cell(in membrane of host cell,not harmful).Rz is also know for the enzyme attack outer membrane with R gene.<br> | ||
Using this system, we cannot only kill target bacteria which are conjugated but also be harmless for other organism.<br><br> | Using this system, we cannot only kill target bacteria which are conjugated but also be harmless for other organism.<br><br> | ||
- | </td><td width=" | + | </td><td width="300"><img src="https://static.igem.org/mediawiki/2011/b/b8/Tokyo_Metropolitan_holin-endolysin.jpg" width="300"><br><div align="center">Fig.2</div></td></tr></table> |
</td></tr> | </td></tr> |
Revision as of 17:30, 5 October 2011
Killing Device
Concept When bee attack enemy, they sting needle and inject toxin. In Japan, the most cause of death from animal is attacted from bee (especially wasp).Bee is awful animal but taking a hint from it, we propose new system for killing bacteria using conjugation. | ||||
Conjugation
| ||||
Cell Lysis
| ||||
Anti-killer device
| ||||
![]() Figure4: Killing Concept | ||||
Parts Design
| ||||
Result Repress expression of GFP by Anti-killing device
We check sequence of P0440(B0034-C0040-B0010-B0012).But sequence of C0040 was incorrect. Sequence of our P0440 is here. Reference [1]University of Birmingham, Horizontal gene transfer (HGT) in biofilms (http://www.birmingham.ac.uk/schools/biosciences/staff/profile.aspx?ReferenceId=6059) [2]Ry Young, Ing-Nang Wang and William D. Roof. Phages will out: strategies of host cell lysis Trends in microbiology, 2000 Elsevier [3]Maria A. Schumacher, Marshall C. Miller, Steve Grkovic, Melissa H. Brown, Ronald A. Skurray and Richard G. Brennan. 2002 Structural basis for cooperative DNA binding by two dimers of the multidrug-binding protein QacR |