Team:UQ-Australia/Data

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Revision as of 15:26, 5 October 2011

A brief summary of some of our laboratory findings is included below. For more information, see the Project page.

We conducted a heat cycle PCR to determine the optimum conditions for amplifying our araC gene. The lanes here are loaded as follows:

1: 1kb+ ladder

2: Negative control (H2O)

3-7: pBAD33 plasmid at temperatures 50C, 55C, 58C, 60C and 65C.

8: Negative control (H2O)

9-13: pBAD33 plasmid at temperatures 50C, 55C, 58C, 60C and 65C.

The expected 900bp band for araC is seen at each temperature

PCR of the lacI gene showing the expected ~1kb band.

PCR of glnG (lanes 2 and 3), glnAp2 (lanes 5 and 6), and the promoter Plac/ara (lanes 2-4 of the second gel). Only glnG is the expected size (1.6kb), while the other two significantly smaller parts (200-300bp) have not been amplified.

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-|rowspan="2"|A brief summary of some of our laboratory findings is included below. For more information, see the 'project' page.
+|rowspan="2"|A brief summary of some of our laboratory findings is included below. For more information, see the [[Team:UQ-Australia/Project|Project]] page.
 ![[File:UQ-Australia_logo_2011.png|125x125px|link=https://2011.igem.org/Team:UQ-Australia]]
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