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Team:Tokyo Metropolitan/Notebook/S29
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Revision as of 13:49, 5 October 2011
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-Visualization-
-Plasmid extraction
Promoter - suppressed expression system(Am)
-Reining
Above samples
-Make competent cells
F+ and RP4
Cultured to OD600:0.6
Harvested
Suspended with 0.1 M CaCl2
Incubated on ice for 10 min
Harvested and abandoned supernatant
Suspended 0.1 M CaCl2
-Transformation
Promoter - suppressed expression system for F+
oriT - GFP for RP4
