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Team:Tokyo Metropolitan/Notebook/S22
From 2011.igem.org
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OriTr-GFP-OriTr 139.5 ng/μl | OriTr-GFP-OriTr 139.5 ng/μl | ||
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| + | -Electrophoresis | ||
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| + | Samples which digested one day ago | ||
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| + | -Gel extraction | ||
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| + | Above samples | ||
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| + | -Ligation | ||
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| + | Promoter - suppressed expression system | ||
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| + | |||
| + | -Transformation | ||
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| + | Above samples | ||
= -Targetting- = | = -Targetting- = | ||
Latest revision as of 13:37, 5 October 2011
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-Visualization-
@Colony PCR and electrophoresis pSB1C3+oriTr-GFP
@preculture from master plate of Colony PCR pSB1C3+oriTr-GFP
@plasmid extraction and DNA refine OriTr, OriTr-GFP
@plasmid extraction and DNA refine OriTr-GFP-OriTr
@DNA densitometry
OriTr-GFP 264.9 ng/μl
OriTr 126.9 ng/μl
OriTr-GFP-OriTr 139.5 ng/μl
-Electrophoresis
Samples which digested one day ago
-Gel extraction
Above samples
-Ligation
Promoter - suppressed expression system
-Transformation
Above samples
-Targetting-
@Colony PCR J23100-P0140
@electrophoresis J23100-K317037 yesterday emzimed and find binds
@gel extraction, but failure
@electrophoresis J23100-P0140, colony PCR find binds
@preculture J23100-P0140, J23100-K317037
