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Team:Tokyo Metropolitan/Notebook/S06
From 2011.igem.org
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Spread no. 26 and no.31 for liquid medium with ampicillin from masterplate of J23100-P0440 on 5th September. After, had been incubated at 37 deg C. | Spread no. 26 and no.31 for liquid medium with ampicillin from masterplate of J23100-P0440 on 5th September. After, had been incubated at 37 deg C. | ||
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| + | = -Visualization- = | ||
| + | -Digestion | ||
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| + | Promoter: Spe1 | ||
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| + | Suppressed expression system: Xba1 and Spe1 | ||
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| + | -Electrophoresis | ||
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| + | Above samples | ||
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| + | -Gel extraction | ||
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| + | Above samples | ||
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| + | -Electrophoresis to check | ||
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| + | Above samples | ||
Latest revision as of 13:21, 5 October 2011
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-Speeding Up-
Colony PCR of HNS(wt)+I20260. Confirmed amplifing DNA but length of fragment was incorrect.
Electrophoresis of digested HNS(wt)(XbaI+SpeI),I20260(BlnⅠ).Concentration of both HNS(wt)(XbaI+SpeI) and I20260(BlnⅠ) was low.
Preculture(pSB1C3)
-Targeting-
-Colony PCR
Did colony PCR for J23100-P0440.
Did not show bands.
-Ligation
Ligated for J23100-K317037 and J23100-P0440.
-Transformation
Transformed for J23100-K317037, J23100-P0440 and J23100 (control).
-Preculture
Spread no. 26 and no.31 for liquid medium with ampicillin from masterplate of J23100-P0440 on 5th September. After, had been incubated at 37 deg C.
-Visualization-
-Digestion
Promoter: Spe1
Suppressed expression system: Xba1 and Spe1
-Electrophoresis
Above samples
-Gel extraction
Above samples
-Electrophoresis to check
Above samples
