Team:Tokyo Metropolitan/Notebook/S07

From 2011.igem.org

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(Speeding Up)
(-Speeding Up-)
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= -Speeding Up- =
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Plasmid extraction and purification (pSB1C3).
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Extracted plasmid from E.coli transformated BBa_J04450,
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Digest HNS(wt) and pSB1C3 with XbaI and SpeI.
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Refined by phenol chloroform mixture and ethanol precipitation,
 +
 
 +
Added restriction enzyme,
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HNS(wt)→X,S
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 +
BBa_J04450→X,S

Revision as of 11:32, 5 October 2011

-Speeding Up-

Extracted plasmid from E.coli transformated BBa_J04450,

Refined by phenol chloroform mixture and ethanol precipitation,

Added restriction enzyme,

HNS(wt)→X,S

BBa_J04450→X,S