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Team:Tokyo Metropolitan/Notebook/S16
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(Created page with "{{:Team:Tokyo_Metropolitan/Header}} <html> <div style="width: 800px; margin-left: 70px; padding-top: 25px; padding-left: 20px;"> </html> = -Visualization- = @plasmid extraction...")
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Revision as of 11:15, 5 October 2011
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-Visualization-
@plasmid extraction oriTr-GFP-oriTr and JM109
@plasmid extraction and DNA refine oriTr-GFP-oriTr and JM109
@DNA densitometry
oriTr-GFP-oriTr 5 26.1 ng/μl
oriTr-GFP-oriTr 6 51.3 ng/μl
oriTr-GFP-oriTr 7 47.2 ng/μl
oriTr-GFP-oriTr 8 58.4 ng/μl
@restriction enzyme to oriTr-GFP-oriTr (XbaⅠ, PstⅠ)
@electrophoresis and gel extraction oriTr-GFP-oriTr5～8 and pSB1C3(restriction enzymed 9/15)
@DNA densitometry
totaled oriTr-GFP-oriTr5,6,8 18.4 ng/μl
pSB1C3① 16.5 ng/μl
pSB1C3② 11.5 ng/μl
@electrophoresis for confirmation, but could not find bind.
