"
Team:Tokyo Metropolitan/Notebook/S12
From 2011.igem.org
(Created page with "{{:Team:Tokyo_Metropolitan/Header}} <html> <div style="width: 800px; margin-left: 70px; padding-top: 25px; padding-left: 20px;"> </html> = -Targeting- = -Electrophoresis Checke...")
Newer edit →
Revision as of 10:24, 5 October 2011
 |
 |
 |
 |
 |
 |
 |
 |
-Targeting-
-Electrophoresis Checked to treat restriction enzyme for J04450 and J23100-P0440. Cut only J23100-P0440.
-Plasmid extraction and DNA refining
Extracted plasmids and refined DNA for J23100-K317037, J23100-P0440, K283030 and J22005.
Results (concentration = ng/ul)
J2310-K317037 (Jack) was 60
J23100-P0440 (Jamper) was 120
K283030 (Kasuga) was 155
J22005 (Jin) was 183
-Treat restriction enzyme
Treated restriction enzyme with E and P for J04450. After, incubated at 37 deg C for 5 hours.
-Electrophoresis
Checked to treat restriction enzyme for J04450. Results was a failure.
-Treat restriction enzyme
Jack -> S and P
Jamper -> E and P
Kasuga -> X and P
Jin -> Nhe
had been incubated at 37 deg C after treat restriction enzyme with preceding enzymes.
