Team:Tokyo Metropolitan/Notebook/S06
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Colony PCR of HNS(wt)+I20260. Confirmed amplifing DNA but length of fragment was incorrect. | Colony PCR of HNS(wt)+I20260. Confirmed amplifing DNA but length of fragment was incorrect. | ||
Revision as of 10:12, 5 October 2011
-Speeding Up-
Colony PCR of HNS(wt)+I20260. Confirmed amplifing DNA but length of fragment was incorrect.
Electrophoresis of digested HNS(wt)(XbaI+SpeI),I20260(BlnⅠ).Concentration of both HNS(wt)(XbaI+SpeI) and I20260(BlnⅠ) was low.
Preculture(pSB1C3)