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Team:Osaka/week8
From 2011.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
| - | ==September 18(Sun)== | + | ==September 18 (Sun)== |
# Miniprep of yesterday's culture | # Miniprep of yesterday's culture | ||
# Restriction digests of minipreppped parts | # Restriction digests of minipreppped parts | ||
| Line 8: | Line 8: | ||
# To liquid culture:03,105,106,107,108,109,110,111,112 | # To liquid culture:03,105,106,107,108,109,110,111,112 | ||
| - | ==September 19(Mon)== | + | ==September 19 (Mon)== |
# Miniprep of yesterday's culture | # Miniprep of yesterday's culture | ||
# Restriction digests of minipreppped parts | # Restriction digests of minipreppped parts | ||
| Line 20: | Line 20: | ||
| - | ==September 20(Tue)== | + | ==September 20 (Tue)== |
# To liquid culture:113,114 | # To liquid culture:113,114 | ||
# colony check (cell survival) | # colony check (cell survival) | ||
| - | ==September 21(Wed)== | + | ==September 21 (Wed)== |
# Miniprep of yesterday's culture | # Miniprep of yesterday's culture | ||
# Restriction digests of minipreppped parts | # Restriction digests of minipreppped parts | ||
| Line 36: | Line 36: | ||
| - | ==September 22(Thu)== | + | ==September 22 (Thu)== |
# Colony check | # Colony check | ||
# Plating | # Plating | ||
# To liquid culture | # To liquid culture | ||
| - | ==September 23(Fri)== | + | ==September 23 (Fri)== |
# Miniprep of yesterday's culture | # Miniprep of yesterday's culture | ||
# Restriction digests of minipreppped parts | # Restriction digests of minipreppped parts | ||
| Line 48: | Line 48: | ||
# Colony check | # Colony check | ||
| - | ==September 24(Sat)== | + | ==September 24 (Sat)== |
# Preparation of LB plates | # Preparation of LB plates | ||
# Plating | # Plating | ||
Revision as of 13:51, 2 October 2011
Contents |
September 18 (Sun)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- To liquid culture:03,105,106,107,108,109,110,111,112
September 19 (Mon)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- ok
- Ligation
- 113(T):105(upstream)+110(downstream)+1-7A(vector)
- 114(T):111(upstream)+112(downstream)+1-7A(vector)
- Transformation of ligation products
- Plating (cell survival)
September 20 (Tue)
- To liquid culture:113,114
- colony check (cell survival)
September 21 (Wed)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- ok
- Ligation
- 115(A):113(upstream)+114(downstream)+1-1G(vector)
- Transformation of ligation products
- colony check (cell survival)
- Plating (cell survival)
September 22 (Thu)
- Colony check
- Plating
- To liquid culture
September 23 (Fri)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- ok
- Colony check
September 24 (Sat)
- Preparation of LB plates
- Plating
