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Team:Osaka/week4
From 2011.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
| - | ==August 21(Sun)== | + | ==August 21 (Sun)== |
# Preparation of LB agar plates (14Tet) | # Preparation of LB agar plates (14Tet) | ||
# Transfer to liquid culture:01,02,03,04 | # Transfer to liquid culture:01,02,03,04 | ||
| Line 13: | Line 13: | ||
|} | |} | ||
| - | ==August 22(Mon)== | + | ==August 22 (Mon)== |
# Colony check | # Colony check | ||
#* Transformed cells produced colonies! | #* Transformed cells produced colonies! | ||
| Line 19: | Line 19: | ||
# Transfer to liquid culture:1-7A | # Transfer to liquid culture:1-7A | ||
| - | ==August 23(Tue)== | + | ==August 23 (Tue)== |
# Miniprep of yesterday's culture | # Miniprep of yesterday's culture | ||
# Restriction digests of minipreppped parts | # Restriction digests of minipreppped parts | ||
| Line 25: | Line 25: | ||
#* 1-7A ok | #* 1-7A ok | ||
| - | ==August 24(Wed)== | + | ==August 24 (Wed)== |
# Gel electrophoresis of 1-7A | # Gel electrophoresis of 1-7A | ||
# Gel extraction of 1-7A | # Gel extraction of 1-7A | ||
| Line 38: | Line 38: | ||
|} | |} | ||
| - | ==August 25(Thu)== | + | ==August 25 (Thu)== |
# Transfer to liquid culture:08,4-15M | # Transfer to liquid culture:08,4-15M | ||
| - | ==August 26(Fri)== | + | ==August 26 (Fri)== |
# Miniprep of yesterday's culture | # Miniprep of yesterday's culture | ||
# Restriction digests of minipreppped parts | # Restriction digests of minipreppped parts | ||
| Line 53: | Line 53: | ||
# Transfer to liquid culture:08 | # Transfer to liquid culture:08 | ||
| - | ==August 27(Sat)== | + | ==August 27 (Sat)== |
# Miniprep of yesterday's culture | # Miniprep of yesterday's culture | ||
# Restriction digests of minipreppped parts | # Restriction digests of minipreppped parts | ||
Revision as of 13:49, 2 October 2011
Contents |
August 21 (Sun)
- Preparation of LB agar plates (14Tet)
- Transfer to liquid culture:01,02,03,04
- Transformaton of Registry parts (See Table 4).
| ID | Part Name | Resistance | Description |
|---|---|---|---|
| 1-7A | <bbpart>BBa_J04450</bbpart> | T | construction plasmid containing mRFP coding device |
August 22 (Mon)
- Colony check
- Transformed cells produced colonies!
- Non-transformed cells (negative controls) did not grow on Tet plates -> confirmed lack of natural antibiotic resistance
- Transfer to liquid culture:1-7A
August 23 (Tue)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- 1-7A ok
August 24 (Wed)
- Gel electrophoresis of 1-7A
- Gel extraction of 1-7A
- Ligation
- 08(T):07(upstream)+1-23L(downstream)+1-7A(vector)
- Transformation of 08 and Registry parts (see Table5)
| ID | Part Name | Resistance | Description |
|---|---|---|---|
| 4-15M | <bbpart>BBa_K325101</bbpart> | C | EPIC Firefly Luciferase Photinus Pyralis (E. coli optimised) |
August 25 (Thu)
- Transfer to liquid culture:08,4-15M
August 26 (Fri)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- 08 ×
- 4-15M ok
- Ligation
- 09(K):1-9N(upstream)+4-15M(downstream)+1-5A(vector)
- 10(K):1-1D(upstream)+4-15M(downstream)+1-5A(vector)
- Transfer to liquid culture:08
August 27 (Sat)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- 08 ok
- Ligation
- 11(A):08(upstream)+06(downstream)+1-1G(vector)
- Transformation of 09,10,11
- Transfer to liquid culture:04
