Team:Osaka/week4

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Revision as of 12:51, 2 October 2011


Contents

August 21(Sun)

  1. Preparation of LB agar plates (14Tet)
  2. Transfer to liquid culture:01,02,03,04
  3. Transformaton of Registry parts (See Table 4).
Table4
IDPart NameResistanceDescription
1-7A<bbpart>BBa_J04450</bbpart>T construction plasmid containing mRFP coding device

August 22(Mon)

  1. Colony check
    • Transformed cells produced colonies!
    • Non-transformed cells (negative controls) did not grow on Tet plates -> confirmed lack of natural antibiotic resistance
  2. Transfer to liquid culture:1-7A

August 23(Tue)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • 1-7A ok

August 24(Wed)

  1. Gel electrophoresis of 1-7A
  2. Gel extraction of 1-7A
  3. Ligation
    • 08(T):07(upstream)+1-23L(downstream)+1-7A(vector)
  4. Transformation of 08 and Registry parts (see Table5)
Table5
IDPart NameResistanceDescription
4-15M<bbpart>BBa_K325101</bbpart>C EPIC Firefly Luciferase　Photinus Pyralis　(E. coli optimised)

August 25(Thu)

  1. Transfer to liquid culture:08,4-15M


August 26(Fri)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • 08 ×
    • 4-15M ok
  4. Ligation
    • 09(K):1-9N(upstream)+4-15M(downstream)+1-5A(vector)
    • 10(K):1-1D(upstream)+4-15M(downstream)+1-5A(vector)
  5. Transfer to liquid culture:08

August 27(Sat)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • 08 ok
  4. Ligation
    • 11(A):08(upstream)+06(downstream)+1-1G(vector)
  5. Transformation of 09,10,11
  6. Transfer to liquid culture:04


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