Team:Macquarie Australia

From 2011.igem.org

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Revision as of 11:25, 30 September 2011

Welcome

G'day from the 2011 Macquarie University iGEM group.

This year's research team will be expanding upon the research conducted by last year's iGEM team. This project involves the production of a phytochrome light switch that jumps between two different light states, acting as a reporter for ambient light conditions. We've nicknamed our work 'the switch-a-roo' as our phytochromes hop back and forth between green and blue states.

Here are some quick links to help you get started:

An overview of our Project

Head on over to our Data Page for a summary of our registered parts

Or meet the Team!

The requirements for each medal grade can be seen at the bottom of this page

Abstract

The objective in this project is to build and characterise a biological light switch in E.coli. This will involve construction of heme-oxygenase and bacteriophytochrome BioBrick parts. In 2010 the Macquarie Team cloned bacteriophytochrome from two sources. They showed that when one was expressed, it was functionally assembled when incubated with exogenous biliverdin; able to elicit a colour change when excited with far-red light. However, the part created is not directly usable as a BioBrick as it contains an internal EcoRI site (Deinococcus radiodurans phytochrome) and 2 PstI sites (Agrobacterium tumefaciens phytochrome). As the phytochromes require binding of biliverdin to work, which is not native to E. coli, the addition of heme oxygenase is required for the production of bilivedin in E.coli. The team from last year had managed to combine a ribosome binding site (RBS) to all 3 proteins of interest. Hence, this year, we aim to convert this 3 RBS-coupled protein coding region into usable BioBricks, and couple these BioBricks together to construct our light switch.

Medal Progress

Bronze	Silver
Registration of Team	Characterisation of our working Biobrick
Judging form	Information entered onto Main Page of Registry
Project wiki
Poster and Talk for Asia Jamboree
Submission of parts to Registry
Submission of Biobrick to Registry [number needed]

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 <br>
-<p style="font: 11pt Trebuchet MS">The objective in this project is to build and characterise a biological light switch in E.coli. This will involve construction of bacteriophytochrome biobrick parts and heme-oxygenase biobrick parts. In 2010 the Macquarie Team cloned bacteriophytochrome from two sources. They showed that when one was expressed that it was functionally assembled when incubated with biliverdin. The part created is not directly usable as a biobrick as it contains an internal PstI site and the XbaI biobrick site is missing. The heme-oxygenase clone also contains an internal restriction site which is not compatible with biobrick assembly.</p>
+<p style="font: 11pt Trebuchet MS">The objective in this project is to build and characterise a biological light switch in E.coli. This will involve construction of heme-oxygenase and bacteriophytochrome BioBrick parts. In 2010 the Macquarie Team cloned bacteriophytochrome from two sources. They showed that when one was expressed, it was functionally assembled when incubated with exogenous biliverdin; able to elicit a colour change when excited with far-red light. However, the part created is not directly usable as a BioBrick as it contains an internal EcoRI site (Deinococcus radiodurans phytochrome) and 2 PstI sites (Agrobacterium tumefaciens phytochrome). As the phytochromes require binding of biliverdin to work, which is not native to E. coli, the addition of heme oxygenase is required for the production of bilivedin in E.coli. The team from last year had managed to combine a ribosome binding site (RBS) to all 3 proteins of interest. Hence, this year, we aim to convert this 3 RBS-coupled protein coding region into usable BioBricks, and couple these BioBricks together to construct our light switch. </p>
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