Team:Macquarie Australia

From 2011.igem.org

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<td class="middle"><a href="https://2011.igem.org/Team:Amsterdam/Labwork/Characterization"><img src="http://governmentconstructioncontracts.com/images/checkbox_green-checkmark.gif">Biobrick submission to Registry</a></td>
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Revision as of 06:37, 30 September 2011




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Welcome


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G'day from the 2011 Macquarie University iGEM group.

This year's research team will be expanding upon the research conducted by last year's iGEM team. This project involves the production of a phytochrome light switch that jumps between two different light states, acting as a reporter for ambient light conditions. We've nicknamed our work 'the switch-a-roo' as our phytochromes hop back and forth between green and blue states.


Here are some quick links to help you get started:

  • An overview of our Project

  • Head on over to our Data Page for a summary of our registered parts

  • Or meet the Team!


Abstract


The objective in this project is to build and characterise a biological light switch in E.coli. This will involve construction of bacteriophytochrome biobrick parts and heme-oxygenase biobrick parts. In 2010 the Macquarie Team cloned bacteriophytochrome from two sources. They showed that when one was expressed that it was functionally assembled when incubated with biliverdin. The part created is not directly usable as a biobrick as it contains an internal PstI site and the XbaI biobrick site is missing. The heme-oxygenase clone also contains an internal restriction site which is not compatible with biobrick assembly.



Medal Progress

Bronze
Silver
Registration of Team Our working Biobrick
Judging form Biobrick submission to Registry
Project wiki
Poster and Talk for Asia Jamboree
Submission of parts to Registry
Poster and Talk for Asia Jamboree
Submission of our New Biobrick

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