Team:USC/Notebook/Week13
From 2011.igem.org
Kevinle1992 (Talk | contribs) |
|||
Line 34: | Line 34: | ||
<table> | <table> | ||
<tr> | <tr> | ||
- | <h1 style="font-family:Verdana;font-weight:700;"> | + | <h1 style="font-family:Verdana;font-weight:700;">Laboratory Notebook</h1> |
</tr> | </tr> | ||
Line 40: | Line 40: | ||
[[Team:USC/Notebook/Brainstorming|Brainstorming]] | [[Team:USC/Notebook/Brainstorming|Brainstorming]] | ||
<br/> | <br/> | ||
- | [[File:Brain_storm.JPG]] | + | [[File:Brain_storm.JPG|link=Team:USC/Notebook/Brainstorming|Brainstorming]] |
</td> | </td> | ||
Line 46: | Line 46: | ||
[[Team:USC/Notebook/Week1|Week 1]] | [[Team:USC/Notebook/Week1|Week 1]] | ||
<br/> | <br/> | ||
- | [[File:week1.jpg]] | + | [[File:week1.jpg|link=Team:USC/Notebook/Week1|Week 1]] |
</td> | </td> | ||
Line 52: | Line 52: | ||
[[Team:USC/Notebook/Week2|Week 2]] | [[Team:USC/Notebook/Week2|Week 2]] | ||
<br/> | <br/> | ||
- | [[File:week2.jpg]] | + | [[File:week2.jpg|link=Team:USC/Notebook/Week2|Week 2]] |
</td> | </td> | ||
Line 58: | Line 58: | ||
[[Team:USC/Notebook/Week3|Week 3]] | [[Team:USC/Notebook/Week3|Week 3]] | ||
<br/> | <br/> | ||
- | [[File:week3.jpg]] | + | [[File:week3.jpg|link=Team:USC/Notebook/Week3|Week 3]] |
</td> | </td> | ||
Line 64: | Line 64: | ||
[[Team:USC/Notebook/Week4|Week 4]] | [[Team:USC/Notebook/Week4|Week 4]] | ||
<br/> | <br/> | ||
- | [[File:week4.jpg]] | + | [[File:week4.jpg|link=Team:USC/Notebook/Week4|Week 4]] |
</td> | </td> | ||
Line 72: | Line 72: | ||
[[Team:USC/Notebook/Week5|Week 5]] | [[Team:USC/Notebook/Week5|Week 5]] | ||
<br/> | <br/> | ||
- | [[File:week5.jpg]] | + | [[File:week5.jpg|link=Team:USC/Notebook/Week5|Week 5]] |
</td> | </td> | ||
Line 78: | Line 78: | ||
[[Team:USC/Notebook/Week6|Week 6]] | [[Team:USC/Notebook/Week6|Week 6]] | ||
<br/> | <br/> | ||
- | [[File:week6.jpg]] | + | [[File:week6.jpg|link=Team:USC/Notebook/Week6|Week 6]] |
</td> | </td> | ||
Line 84: | Line 84: | ||
[[Team:USC/Notebook/Week7|Week 7]] | [[Team:USC/Notebook/Week7|Week 7]] | ||
<br/> | <br/> | ||
- | [[File:week7.jpg]] | + | [[File:week7.jpg|link=Team:USC/Notebook/Week7|Week 7]] |
</td> | </td> | ||
Line 90: | Line 90: | ||
[[Team:USC/Notebook/Week8|Week 8]] | [[Team:USC/Notebook/Week8|Week 8]] | ||
<br/> | <br/> | ||
- | [[File:week8.jpg]] | + | [[File:week8.jpg|link=Team:USC/Notebook/Week8|Week 8]] |
</td> | </td> | ||
Line 96: | Line 96: | ||
[[Team:USC/Notebook/Week9|Week 9]] | [[Team:USC/Notebook/Week9|Week 9]] | ||
<br/> | <br/> | ||
- | [[File:week9.jpg]] | + | [[File:week9.jpg|link=Team:USC/Notebook/Week9|Week 9]] |
</td> | </td> | ||
Line 104: | Line 104: | ||
[[Team:USC/Notebook/Week10|Week 10]] | [[Team:USC/Notebook/Week10|Week 10]] | ||
<br/> | <br/> | ||
- | [[File:week10.jpg]] | + | [[File:week10.jpg|link=Team:USC/Notebook/Week10|Week 10]] |
</td> | </td> | ||
Line 110: | Line 110: | ||
[[Team:USC/Notebook/Week11|Week 11]] | [[Team:USC/Notebook/Week11|Week 11]] | ||
<br/> | <br/> | ||
- | [[File:week11.jpg]] | + | [[File:week11.jpg|link=Team:USC/Notebook/Week11|Week 11]] |
</td> | </td> | ||
Line 116: | Line 116: | ||
[[Team:USC/Notebook/Week12|Week 12]] | [[Team:USC/Notebook/Week12|Week 12]] | ||
<br/> | <br/> | ||
- | [[File:week12.jpg]] | + | [[File:week12.jpg|link=Team:USC/Notebook/Week12|Week 12]] |
</td> | </td> | ||
Line 122: | Line 122: | ||
[[Team:USC/Notebook/Week13|Week 13]] | [[Team:USC/Notebook/Week13|Week 13]] | ||
<br/> | <br/> | ||
- | [[File:week13.jpg]] | + | [[File:week13.jpg|link=Team:USC/Notebook/Week13|Week 13]] |
</td> | </td> | ||
Line 128: | Line 128: | ||
[[Team:USC/Notebook/Week14|Week 14]] | [[Team:USC/Notebook/Week14|Week 14]] | ||
<br/> | <br/> | ||
- | [[File:week14.jpg]] | + | [[File:week14.jpg|link=Team:USC/Notebook/Week14|Week 14]] |
</td> | </td> | ||
Line 137: | Line 137: | ||
</table> | </table> | ||
+ | |||
<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 13:'''</h3> | <h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 13:'''</h3> | ||
Latest revision as of 03:58, 29 September 2011
|
|||||||||||||||
|
Week 13:
09/06/2011
1. Digest/ ligate casO+PCDF
2. Transform ligated products into BL21 with tetO::GFP
3. Transform CRISPR GFP#7 into BL21 with tetO::GFP
4. Inoculate HT5 with/without IPTG
5. Inoculate HT115+tetO::GFP
6. Purify casO (PCR products)
7. Measure the DNA of casO::PCDF
09/07/2011
1. Make competent cells as follows:
BL21 (tetO::GFP, CRISPR::GFP)
HT115(tetO::GFP, CRISPR::GFP)
HT115(tetO::GFP)
2. Take OD reading of HT115 with/ without IPTG
3. Digest/ligate casO+PCDF
4. Transform CRISPR#7 into tetO cells(BL21)
5. Take equal amount of E.Coli, miniprep, transform into new DH5α, select on amp.
09/19/2011
1. Growth test, grow all the bacteria overnight
2. PCR cas3+casO from gDNA
09/08/2011
1. OD reading
2. Digest/ligate reaction
tetR with pSB1k3
tetO with pSB1k3
casO with PCDF
Transform into DH5α, and also IPTG samples into DH5α
09/09/2011
1. Make BL21(tetO, CRISPR-GFP #7) competent and make freeze stock
2. Make HT115(tetO, CRISPR-GFP #7) competent and make freeze stock
3. Make HT115(CRISPR-GFP #7) competent and make freeze stock