Team:USC/Notebook/Week12

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<h1 style="font-family:Verdana;font-weight:700;">Brainstorming</h1>
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[[Team:USC/Notebook/Week13|Week 13-14]]
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[[Team:USC/Notebook/Week14|Week 14]]
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<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 12:'''</h3>
<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 12:'''</h3>

Revision as of 03:38, 29 September 2011

USC Banner.jpg


Brainstorming

Brainstorming
Brain storm.JPG

Week 1
Week1.jpg

Week 2
Week2.jpg

Week 3
Week3.jpg

Week 4
Week4.jpg

Week 5
Week5.jpg

Week 6
Week6.jpg

Week 7
Week7.jpg

Week 8
Week8.jpg

Week 9
Week9.jpg

Week 10
Week10.jpg

Week 11
Week11.jpg

Week 12
Week12.jpg

Week 13
Week13.jpg

Week 14
Week14.jpg

Week 12:

08/29/2011
1. re-PCR for CASO in PCDF vector
2. Inoculate DH5α, BL21 and HT115 cells in 5mL LB
3. Purified CAS3 (#6,8,9,12)
4. Put CRISPR-tetR into PCOLA (KanR) instead of tetR (CMR)


08/30/2011
1. For CASO
gDNA isolation of DH5α and HT115 cells
PCR CASO from gDNA with primers: AP1+CASOR
2. Inoculate PCOLA+PCDF
3. For CAS3
4. Transform cas3 into tetO+CRISPR-GFP cells. Notes: use controls
5. Gel verify casO
6. Make competent cells of tetO+GFP+CRISPR+cas3
7. Inoculate 6 culture tubes by IPTG or Without IPTG
tetO+GFP+CRISPR+cas3 (#12, 9, 6)
tetO+GFP+CRISPR
8. Transformation teto-GFP or CRISPR-GFP (# 2,4,5,6,7)or tetO-GFP and CRISPR-GFP (#1,2,3,4,5,6,7) into HT115 cells

09/01/2011
1. Inoculation of HT115 with/without IPTG
2. Freeze down both tubes of tetO::GFP CRISPR-GFP (B4) and A1, B1, C1

Retrieved from "http://2011.igem.org/Team:USC/Notebook/Week12"