Team:USC/Notebook/Week5

From 2011.igem.org

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                        <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Attributions|Attributions]]</span></span>
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<h1 style="font-family:Verdana;font-weight:700;">Week 5</h1>
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[[Team:USC/Notebook/Week13|Week 13-14]]
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[[Team:USC/Notebook/Week14|Week 14]]
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<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 5:'''</h3>
<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 5:'''</h3>
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5. PCR CASO by using old primers
5. PCR CASO by using old primers
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Revision as of 03:23, 29 September 2011

USC Banner.jpg


Week 5

Brainstorming
Brain storm.JPG

Week 1
Week1.jpg

Week 2
Week2.jpg

Week 3
Week3.jpg

Week 4
Week4.jpg

Week 5
Week5.jpg

Week 6
Week6.jpg

Week 7
Week7.jpg

Week 8
Week8.jpg

Week 9
Week9.jpg

Week 10
Week10.jpg

Week 11
Week11.jpg

Week 12
Week12.jpg

Week 13
Week13.jpg

Week 14
Week14.jpg

Week 5:

07/05/2011
1. digestion step by step for MET25 plasmid see protocols in general protocols


07/06/2011
1. Purify Gel DNA
2. Run a gell test :
MET25, pRS423-426, tetO+cFP, tetO+RFP, tetO+GFP,CAS3, CASO 3. PCR CASO primers


07/07/2011
1. Transformation for all 4 ligation plasmids:
TPS1, MSN2, ELO1, OLE1+pRS vectors
2. Gel verify for CASO
3. Amplify the verified products by PCR
4. Reisolate the genomic DNA
5. PCR CASO by using old primers

Retrieved from "http://2011.igem.org/Team:USC/Notebook/Week5"