From 2011.igem.org
(Difference between revisions)
|
|
| Line 1: |
Line 1: |
| - | We repeated the PCR of the Biobrick using other master mix, with the miniprep´s products obtained from the Biobrick ordered at MIT.
| |
| | | | |
| - |
| |
| - | We prepared a chloramphenicol stock (50mg/mL), which was stored at -20 °C.
| |
| - |
| |
| - |
| |
| - | Primers used for PCR:
| |
| - |
| |
| - | • RhT2b+RhT2a
| |
| - |
| |
| - | • RhTbio2a+RhTbio2b
| |
| - |
| |
| - | • RhTbioF1b+RhTbioF1a
| |
| - |
| |
| - | • RhT1a+RhT1b
| |
| - |
| |
| - | Program used for PCR:
| |
| - |
| |
| - | [[File:tabla2.png|700px|thumb|left|alt text]]
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | We aliquoted Primers again (work solutions).
| |
| - |
| |
| - | We did an electrophoresis of the PCR´s products on an agarose gel 1% TAE 1X (We hadn´t any results).
| |
| - |
| |
| - | PCR (Probing primer´s effectiveness with a Pseudomonas aeruginosa´s genomic DNA)
| |
| - |
| |
| - | [[File:tabla3.png|700px|thumb|left|alt text]]
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | Volume of master mix: 12.5 uL
| |
| - |
| |
| - | Volume of DNA: 5 uL
| |
| - |
| |
| - | Volume of Primers: 2.5 uL to everyone
| |
| - |
| |
| - | Volume of H2O: 5.5 uL
| |
| - |
| |
| - | Total Volume of reaction: 28 uL
| |
| - |
| |
| - | We hadn´t any results, we realized that Taq Pol wasn´t capable of polymerize fragments most heavy than 1000 bp.
| |
Latest revision as of 03:16, 29 September 2011
"
