Team:Calgary/Notebook/Protocols/Process27

From 2011.igem.org

(Difference between revisions)
(Created page with "{{Team:Calgary/Main_Header|notebook}} {{Team:Calgary/Notebookbar| TITLE=Electrical Settings| BODY=<html> <p>For the electrodes we used the Screen printed Carbon Electrodes from ...")
 
Line 4: Line 4:
TITLE=Electrical Settings|
TITLE=Electrical Settings|
BODY=<html>
BODY=<html>
-
<p>For the electrodes we used the Screen printed Carbon Electrodes from Pine Instruments. These electrodes needed to be plated in zinc phthalocyanine for our purposes.</p><br>
+
<p>For the generation of a standard curve we used a Pin Instruments WaveNow potentiostat. The accompanying software, AfterMath, was used to record the data. Below is a screenshot of the settings used during the experiment.</p><br>
-
<ol>
+
<img style="width:450px; display:block; margin-left:auto; margin-right:auto;" src="https://static.igem.org/mediawiki/2011/6/68/UofC_Electrical_2011.jpg"></img><br>
-
<li>Dissolve 100µg of zinc phthalocyanine in 10mL of DMF.</li>
+
<p>This range will accomodate the oxidation of chlorophenol red and give enough room to generate a sufficient standard curve. The 6 segments means that the data generated is in triplicate. Varying the sweep rate will alter the resolution of the graph, with smaller sweep rates causing higher resolutions.</p>
-
<li>Stir until dissolved.</li>
+
-
<li>Dip only the working electrode (outer electrode) into the solution, and hold it there for 5 seconds.</li>
+
-
<li>Let the electrode air dry until there is no more solution present on the electrode.</li>
+
-
</ol><br>
+
-
<p><b>NOTE</b>: This should be performed in a fume hood due to the toxic nature of DMF</p>
+
</html>
</html>
}}
}}

Latest revision as of 03:15, 29 September 2011


Electrical Settings

For the generation of a standard curve we used a Pin Instruments WaveNow potentiostat. The accompanying software, AfterMath, was used to record the data. Below is a screenshot of the settings used during the experiment.



This range will accomodate the oxidation of chlorophenol red and give enough room to generate a sufficient standard curve. The 6 segments means that the data generated is in triplicate. Varying the sweep rate will alter the resolution of the graph, with smaller sweep rates causing higher resolutions.