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Team:Panama/16 June 2011
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Revision as of 02:52, 29 September 2011
We autoclaving 1.5ml eppendorff tubes and then were treat at 800C for 2h in the microwave for nuclease elimination. Orlando prepare chloranfenicol (100µg/ml) We pass our Biobrick from Agar medium +chloranfenicol to LB medium + Chloranfenicol. Sandra use as negative control : 200µl plasmid (RBS+T) in medium with Amp and inoculate in LB medium with Chloranfenicol. No colonies have to grow because not have resistance for chloranfenicol Incubate at 370 C in shaker.
