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Team:Panama/1 June 2011
From 2011.igem.org
(Difference between revisions)
(Created page with "We did the transformation of P+R and G+T with negative controls. Transformation protocol material: Ice PCR tubes EPP tubes Thermocycler SOC medium Micropipette peaks For the pr...") |
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Transformation protocol material: | Transformation protocol material: | ||
| - | Ice | + | Ice, PCR tubes, EPP tubes, Thermocycler,SOC medium,Micropipette peaks |
| - | PCR tubes | + | |
| - | EPP tubes | + | |
| - | Thermocycler | + | |
| - | SOC medium | + | |
| - | Micropipette peaks | + | |
For the process of transformation we used the IGEM protocol. | For the process of transformation we used the IGEM protocol. | ||
We prepared LB medium + agar and inoculate bacteria on petri dishes. | We prepared LB medium + agar and inoculate bacteria on petri dishes. | ||
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| + | Negative control (C-) don’t have to grow because doesn’t have the resistance plasmid for ampicillin the antibiotic that we use. The petri dishes have 0.2 µl of Amp. | ||
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| + | We check those petri dishes in the evening and growth it well but negative control grows too, therefore, have to do the transformation again. We think that the reason maybe LB medium has contaminate because wasn’t autoclaving. | ||
Latest revision as of 02:45, 29 September 2011
We did the transformation of P+R and G+T with negative controls.
Transformation protocol material: Ice, PCR tubes, EPP tubes, Thermocycler,SOC medium,Micropipette peaks
For the process of transformation we used the IGEM protocol. We prepared LB medium + agar and inoculate bacteria on petri dishes.
Negative control (C-) don’t have to grow because doesn’t have the resistance plasmid for ampicillin the antibiotic that we use. The petri dishes have 0.2 µl of Amp.
We check those petri dishes in the evening and growth it well but negative control grows too, therefore, have to do the transformation again. We think that the reason maybe LB medium has contaminate because wasn’t autoclaving.
