Team:Arizona State/Notebook/September

From 2011.igem.org

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:* sample appears slightly higher than expected
:* sample appears slightly higher than expected
::* some distortion and low resolution
::* some distortion and low resolution
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== Sunday, September 4 ==
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== Sunday, September 4 to Friday, September 9 ==
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== Monday, September 5 ==
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== Tuesday, September 6 ==
+
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== Wednesday, September 7 ==
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== Thursday, September 8 ==
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== Friday, September 9 ==
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* cas9 PCR products restricted overnight
* cas9 PCR products restricted overnight
== Saturday, September 10 ==
== Saturday, September 10 ==
* 9/10 restriction products dephosphorylated for 2 hours
* 9/10 restriction products dephosphorylated for 2 hours
* Overnight ligation of dephosphorylated restriction products
* Overnight ligation of dephosphorylated restriction products
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== Sunday, September 11 ==
+
== Sunday, September 11 to Saturday, September 17 ==
* 9/8 restriction - 9/10 ligation products transformed into NEB cells  
* 9/8 restriction - 9/10 ligation products transformed into NEB cells  
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== Monday, September 12 ==
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== Sunday, September 18 to Saturday, September 24 ==
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== Tuesday, September 13 ==
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*
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== Sunday, September 25 to Wednesday, September 28 ==
*
*
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== Wednesday, September 14 ==
 
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== Thursday, September 15 ==
 
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== Friday, September 16 ==
 
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== Saturday, September 17 ==
 
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== Sunday, September 18 ==
 
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== Monday, September 19 ==
 
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== Tuesday, September 20 ==
 
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== Wednesday, September 21 ==
 
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== Thursday, September 22 ==
 
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== Friday, September 23 ==
 
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== Saturday, September 24 ==
 
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== Sunday, September 25 ==
 
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== Monday, September 26 ==
 
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== Tuesday, September 27 ==
 
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== Wednesday, September 28 ==
 
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Revision as of 02:26, 29 September 2011


Notebook: September


ASU Logo.png
 

Thursday, September 1

  • Started to develop Listeria Innocua project component.
  • Listeria CRISPR only uses cas9 tracr gene
  • Project requires:
  • cas9
  • Duet plasmid
  • Listeria T7 RSR
  • Ligate all together in biobrick
  • Test with protospacer

Saturday, September 3

  • 3 PCR reactions using LI as template
  • Samples visualized on gel
  • sample appears slightly higher than expected
  • some distortion and low resolution

Sunday, September 4 to Friday, September 9

  • cas9 PCR products restricted overnight

Saturday, September 10

  • 9/10 restriction products dephosphorylated for 2 hours
  • Overnight ligation of dephosphorylated restriction products

Sunday, September 11 to Saturday, September 17

  • 9/8 restriction - 9/10 ligation products transformed into NEB cells

Sunday, September 18 to Saturday, September 24

Sunday, September 25 to Wednesday, September 28