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Team:Calgary/Notebook/Protocols
From 2011.igem.org
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<h2>Pseudomonas Biotinylation Techniques</h2> | <h2>Pseudomonas Biotinylation Techniques</h2> | ||
| - | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process1">Plasmid | + | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process1">Plasmid Isolation (from <i>Pseudomonas spp.</i>)</a></li> |
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process22">Biotinylation/Immunoprecipitation of Naphthenic Acids</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process22">Biotinylation/Immunoprecipitation of Naphthenic Acids</a></li> | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process23">Conjugation of <i>E. coli</i> plasmids to <i>Pseudomonas spp.</i>.</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process23">Conjugation of <i>E. coli</i> plasmids to <i>Pseudomonas spp.</i>.</a></li> | ||
Revision as of 01:55, 29 September 2011
Notebook
Post-Regionals
Journal
- Defining Objectives
- Techniques Workshop
- Bioinformatics & qRT-PCR
- Sensory Element Fishing
- Electrochem - CPR Oxidation
- Electrochem - Reporter Gene
- Pseudomonas Tools
- Microalgae Tools
- NA Viability Assays
- May - July Group Journal
Protocols
Safety
Protocols
Here is a list of the procedures we used this summer. Each one has a description and list of materials needed.
- Preparation of Algal f2 Media
- Glass Bead Algal transformation
- Chloroplast Isolation
- Nuclear DNA extraction from Algae
- Plasmid Isolation (from Pseudomonas spp.)
- Biotinylation/Immunoprecipitation of Naphthenic Acids
- Conjugation of E. coli plasmids to Pseudomonas spp..
- Plasmid Isolation (from E. coli)
- RNA Extraction
- cDNA Synthesis
- Quantitative Real Time PCR
- Testing the pBAD-LacZ Construct
- Taq PCR Protocol
- PCR Purification
- Gel Extraction
- Testing the LacI-LacZ I732901 gene
- Bacterial Transformation
- Rehydration of Registry DNA
- Construction Techniques
- Agarose Gel Electrophresis
- LB Agar Plates
- Overnight Cultures
- Preparing chemically competent cells (E. coli)
- Preparing glycerol stocks (E. coli)
Microalgae Protocols
Pseudomonas Biotinylation Techniques
General Protocols
