Team Acknowledgements

Our project was conceived and designed by the Harvard iGEM 2011 advisors and mentors, including but not limited to, Jagesh Shah, Srivatsan Raman, Daniel Goodman, Noah Taylor, Jameson Rogers, Joyce Yang, and Jun Li. The project was made possible by support from the George Church lab at the Wyss Institute for Biologically Inspired Engineering as well as Alain Viel at the Harvard Department of Molecular and Cellular Biology. Without their valuable guidance and great patience, we never would have been able to achieve what have done so far.

Although the project is the first to utilize several key technologies in novel ways, the following technologies were developed outside of iGEM. The multiplex automated genome engineering (MAGE) method was developed by Harris Wang et al in the Church lab. The chip-based synthesis method that we used to synthesize oligos for our zinc finger pool was developed by Sri Kosuri et al in the Church lab, and the actual oligo synthesis was generously provided by Agilent Technologies.

We owe great thanks to Alain Viel for his flawless management of the logistics of iGEM, including but not limited to providing lab space, ordering needed lab supplies, arranging our trip to the Americas Regional, and for his good taste in footware. Furthermore, we would also like to thank our lab manager Andrew Cumming for all of his practical lab advice and for helping to make everything run smoothly in the day to day logistics of lab.

Team Member Acknowledgements

All project work was done by team members, and we would like to make the following acknowledgements:

Kristin Barclay Bioinformatics, chip sequences design and programming, majority of wiki coding and design

Justin Chew Bioinformatics, ZF target selection, ZF expression plasmid construction, Wolfe selection system testing

William Clerx Bioinformatics, ZF target selection, wiki coding and design, video editing, freelance wet lab

Sarah Choudhury TolC selection system construction, TolC and Wolfe selection system testing, biobrick construction

Naomi Genuth Wolfe selection system construction and testing, biobrick construction and registration

Brandon Gerberich Bioinformatics, Wolfe selection system construction and testing, interview cameraman and video editor

Mark Kopelman Wet lab, video editing

Matt Lunati TolC selection system construction and testing

Nida Naushad Bioinformatics, ZF expression plasmid construction, chip library assembly, biobrick construction

Advisor Acknowledgements

Finally, we would like to specially acknowledge all of our teaching fellows (TFs) who have always been there for us through thick and thin, no matter how small or big the problem. Their advice has been of immeasurable value to our project's success. We would also like to also acknowledge the following TFs for their special contribution:

Daniel Goodman Harvard iGEM logo design, wiki design (contributer to CSS and designer of the navigation bar), and being an all around awesome team leader

Noah Taylor Using lambda-red recombination to successfully change our zinc finger binding sites when MAGE was impractical

Srivatsan Raman Collaborator in our project abstract and Human Practices page