Team:Caltech/Week 3
From 2011.igem.org
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- | == June | + | ==June 26== |
- | <p> | + | <p> Start overnight cultures of T7 polymerase ([http://partsregistry.org/Part:BBa_K145001 K145001]), mCherry ([http://partsregistry.org/Part:BBa_J06702 J06702]), Lac Promoter ([http://partsregistry.org/Part:BBa_R0010 R0010]), double terminator ([http://partsregistry.org/Part:BBa_B0014 B0014] and [http://partsregistry.org/Part:BBa_B0015 B0015]) and Tet Promoter ([http://partsregistry.org/Part:BBa_R0040 R0040])<br/> |
+ | Transfer 0.5 mL aliquots of BPA and 5 mL aliquots 17a-ethynylestradiol cultures from June 23 to fresh minimal media</p> | ||
+ | |||
+ | == This Week == | ||
+ | <p>Ethanol precipitation of DNA extractions from LA river samples<br/> | ||
+ | Miniprep of T7 polymerase ([http://partsregistry.org/Part:BBa_K145001 K145001]), mCherry ([http://partsregistry.org/Part:BBa_J06702 J06702]), Lac Promoter ([http://partsregistry.org/Part:BBa_R0010 R0010]), double terminator ([http://partsregistry.org/Part:BBa_B0014 B0014] and [http://partsregistry.org/Part:BBa_B0015 B0015]) and Tet Promoter ([http://partsregistry.org/Part:BBa_R0040 R0040]) and submit for sequencing<br/> | ||
+ | PCR of parts for pNT001 (Test plasmid for [http://partsregistry.org/Part:BBa_K123000 K123000])<br/> | ||
+ | Purification/Gibson assembly for pNT001<br/> | ||
+ | Cutting LA River DNA for insertion into fosmids<br/> | ||
+ | Email other teams <br/> | ||
+ | Insert LA River DNA into fosmids <br/> | ||
+ | Transform fosmids into E. coli and start enrichment cultures<br/> | ||
+ | Continue gene fishing <br/> | ||
+ | Send off continued forward sequencing for HER<br/> | ||
+ | PCR of parts for pNT002 and assembly<br/> | ||
+ | Use pNT001 and pNT002 to test degradation parts</p> | ||
}} | }} |
Revision as of 00:30, 25 June 2011
Project |
June 26 Start overnight cultures of T7 polymerase ([http://partsregistry.org/Part:BBa_K145001 K145001]), mCherry ([http://partsregistry.org/Part:BBa_J06702 J06702]), Lac Promoter ([http://partsregistry.org/Part:BBa_R0010 R0010]), double terminator ([http://partsregistry.org/Part:BBa_B0014 B0014] and [http://partsregistry.org/Part:BBa_B0015 B0015]) and Tet Promoter ([http://partsregistry.org/Part:BBa_R0040 R0040]) This WeekEthanol precipitation of DNA extractions from LA river samples
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