Team:Harvard/Results/Chip Synthesis

From 2011.igem.org

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When performing a qPCR, one wants to run the reaction while the growth is still exponential. During this phase, all the oligos are replicated at equal rates. Once growth levels off, the reaction should be stopped as the oligos are now being replicated unequally. Each of the graphs below represents a qPCR for one of our sub-pools.   
When performing a qPCR, one wants to run the reaction while the growth is still exponential. During this phase, all the oligos are replicated at equal rates. Once growth levels off, the reaction should be stopped as the oligos are now being replicated unequally. Each of the graphs below represents a qPCR for one of our sub-pools.   
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<html>
 
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<script>
 
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function show(k)
 
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{
 
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elem = document.getElementById('GNN');
 
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  elem.style.display = 'none';
 
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if(k==1){
 
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elem.style.display = 'block';
 
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}
 
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elem = document.getElementById('TNN');
 
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  elem.style.display = 'none';
 
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if(k==2){
 
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elem.style.display = 'block';
 
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}
 
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elem = document.getElementById('CNN');
 
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  elem.style.display = 'none';
 
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if(k==3){
 
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elem.style.display = 'block';
 
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}
 
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elem = document.getElementById('ANN');
 
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  elem.style.display = 'none';
 
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if(k==4){
 
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elem.style.display = 'block';
 
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}
 
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elem = document.getElementById('NGN');
 
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  elem.style.display = 'none';
 
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if(k==5){
 
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elem.style.display = 'block';
 
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}
 
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elem = document.getElementById('NTN');
 
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  elem.style.display = 'none';
 
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if(k==6){
 
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elem.style.display = 'block';
 
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}
 
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elem = document.getElementById('0');
 
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  elem.style.display = 'none';
 
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if(k==13){
 
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elem.style.display = 'block';
 
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}
 
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elem = document.getElementById('.01');
 
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  elem.style.display = 'none';
 
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if(k==14){
 
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elem.style.display = 'block';
 
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}
 
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elem = document.getElementById('.015');
 
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  elem.style.display = 'none';
 
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if(k==15){
 
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elem.style.display = 'block';
 
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}
 
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elem = document.getElementById('.02');
 
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  elem.style.display = 'none';
 
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if(k==16){
 
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elem.style.display = 'block';
 
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}
 
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}
 
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</script>
 
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<div id="marginbox">
 
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<table>
 
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<tr><td><span id="student" onclick="show(1)">CB Top</span></td></tr>
 
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<tr><td><span id="student" onclick="show(2)">CB Bot</span></td></tr>
 
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<tr><td><span id="student" onclick="show(3)">FH Top</span></td></tr>
 
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<tr><td><span id="student" onclick="show(4)">FH Bot</span></td></tr>
 
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<tr><td><span id="student" onclick="show(5)">Myc 198</span></td></tr>
 
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<tr><td><span id="student" onclick="show(6)">Myc 981</span></td></tr>
 
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</table>
 
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</div>
 
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</html>
 
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<div id="GNN" style="width:900px">[[File:HARVGnn_freqs.png|frame|left|Probability data for the 783 fingers that bind to '''GNN''' triplets. Note the high probability of leucine at position 4 and arginine at position 6.]]</div>
 
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<div id="TNN" style="display:none">[[File:HARVTnn_probs.png|frame|left|Probability data for the 128 fingers that bind to '''TNN''' triplets. Note the high probability of leucine at position 4.]]</div>
 
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<div id="CNN" style="display:none">[[File:HARVCnn_probs.png|frame|left|Probability data for the 16 fingers that bind to '''CNN''' triplets. There may not be enough data to consider this information statistically significant]]</div>
 
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<div id="ANN" style="display:none">[[File:HARVAnn_probs.png|frame|left|Probability data for the 29 fingers that bind to '''ANN''' triplets. There may not be enough data to consider this information statistically significant]]</div>
 
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<div id="NGN" style="display:none">[[File:HARVNgn_probs.png|frame|left|Probability data for the 298 fingers that bind to '''NGN''' triplets. The position 4 leucine motif remains. There is also a high probability (> 0.5) of a histidine at position 3 and an arginine at position 6.]]</div>
 
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<div id="NTN" style="display:none"> [[File:HARVNtn_probs.png|frame|left|Probability data for the 177 fingers that bind to '''NTN''' triplets. The position 4 leucine motif remains.]]</div>
 
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<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
 
</div>
</div>

Revision as of 18:46, 28 September 2011

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qPCR curves

When performing a qPCR, one wants to run the reaction while the growth is still exponential. During this phase, all the oligos are replicated at equal rates. Once growth levels off, the reaction should be stopped as the oligos are now being replicated unequally. Each of the graphs below represents a qPCR for one of our sub-pools.


Sequencing results of Library transformation

Error rates

Still being updated

  • Good: 57.8% (22/38)
  • Single SNP: 5.3% (2/38)
  • Multiple SNPs: 18.4% (7/38)
  • Frame shift: 18.4% (7/38)

Distributions

Still being updated Each of the sequenced zinc fingers have original F1 fingers.