Team:Arizona State/Lab/Protocols/Transformation

NEB ([http://www.neb.com/nebecomm/products/protocol117.asp 10-beta] and [http://www.neb.com/nebecomm/products/protocol78.asp Turbo])

[http://openwetware.org/wiki/Transforming_chemically_competent_cells TSS]

1. Thaw 50ul prepared competent TSS cells on ice.
2. Add approximately 1 ul prepped plasmid to the thawed cells. Make sure the culture is thoroughly mixed.
3. Incubate for 30 minutes on ice.
4. Incubate for 30 seconds at 42C (in a water bath).
5. Incubate for 2 minutes on ice.
6. Add 1 ml room temperature SOC.
7. Incubate for 1 hour at 37C with shaking.
8. Plate with appropriate antibiotic.
9. Grow overnight at 37C.