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Reporter: Week 11 July 26-30
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(Created page with "==Tuesday, July 26== Clone tev protease (K316017) into K3 ==Wednesday, July 27== All colonies from July 26 clone=red, retry clone tev protease into K3 Clone XylE and GFP into K3...")
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(Created page with "==Tuesday, July 26== Clone tev protease (K316017) into K3 ==Wednesday, July 27== All colonies from July 26 clone=red, retry clone tev protease into K3 Clone XylE and GFP into K3...")
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Revision as of 06:59, 28 September 2011
Contents |
Tuesday, July 26
Clone tev protease (K316017) into K3
Wednesday, July 27
All colonies from July 26 clone=red, retry clone tev protease into K3
Clone XylE and GFP into K3
Prepare cultures of K316009 for catechol assay
Thursday, July 28
Colony PCR GFP+K3, XylE+K3, RecA+K3 and tev protease+K3 Results: GFP+K3 and tev protease+K3 failed, all else worked
Test K316009 with catechol assay Results: no difference between induced and uninduced cultures
Friday, July 29
clone tev protease into K3
J23100+RBS34 GFP+tev and cI cleavage sites
Saturday, July 30
Colony PCR of J23100+RBS34, GFP+tev cleavage site, GFP+cI cleavage site Results: all failed
XylE+3 linkers LacZα+linkers
