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Promoters and Terminators
The iGEM registry is lacking in promoter and terminators sequences for yeast, especially when compared with the number available for use in E. coli. In order to address this problem and increase the use of yeast in synthetic biology we have decided to provide a library of promoter and terminator sequences from 12 yeast genes: 4 causing high expression, 4 causing medium expression, and 4 causing low expression. We hope these BioBrick compatible parts will make it easier for future iGEM teams to work with Saccharomyces cerevisiae.
Promoters
Promoter sequences that have been BioBricked:
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530016 RPS8Bp] | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530005 BAP2p] | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530015 FCY2p] | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530018 Gal 1/10p] | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530010 HHO1p] |
|---|---|---|---|---|
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530003 KRE9p] | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530007 PRY1p] | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530004 STM1p] | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530008 TDH3p] | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530009 RPS2p] |
Terminators
3'UTR sequences that have been BioBricked:
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530020 ARD1utr] | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530006 BAP2utr] | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530017 FCY2utr] | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530019 HHo1utr] | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530014 KRE9utr] |
|---|---|---|---|---|
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530013 PRY1utr] | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530011 RPL8Autr] | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530021 RPL24Autr] | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530024 RPS2utr] | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530022 RPS8Butr] |
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530023 TDH3utr] |
Promoters and 3'UTRs for Golden Gate Assembly
We also have a second promoter/3'UTR library consisting of the same sequences with custom overhangs with BsaI sites rather than the BioBrick sites. These are being used in our two violacein projects, where they will allow Golden Gate assembly of violacein expression cassettes.
