"
Team:Arizona State/Project/L innocua
From 2011.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
__NOTOC__ | __NOTOC__ | ||
| - | == | + | ==Experimental Design== |
This is our experimental flowchart for the L. innocua CRISPR platform. | This is our experimental flowchart for the L. innocua CRISPR platform. | ||
[[Image: LI_Experimental_Flow_chart.png|750px]] | [[Image: LI_Experimental_Flow_chart.png|750px]] | ||
| + | |||
| + | ==Plasmid Design== | ||
The plasmid containing CRISPR derived from Listeria Innocua CLIP11262 contains the standard BioBrick Suffix (SpeI and PstI Sites) at the end of the array distal to the Leader sequence and T7 Promoter, thus Biobrick compatible Repeat Spacer Repeat (RSR) sections can sequentially be added into the vector to expand the CRISPR repertoire. | The plasmid containing CRISPR derived from Listeria Innocua CLIP11262 contains the standard BioBrick Suffix (SpeI and PstI Sites) at the end of the array distal to the Leader sequence and T7 Promoter, thus Biobrick compatible Repeat Spacer Repeat (RSR) sections can sequentially be added into the vector to expand the CRISPR repertoire. | ||
Revision as of 23:40, 27 September 2011
|
|
 Experimental DesignThis is our experimental flowchart for the L. innocua CRISPR platform.
Plasmid DesignThe plasmid containing CRISPR derived from Listeria Innocua CLIP11262 contains the standard BioBrick Suffix (SpeI and PstI Sites) at the end of the array distal to the Leader sequence and T7 Promoter, thus Biobrick compatible Repeat Spacer Repeat (RSR) sections can sequentially be added into the vector to expand the CRISPR repertoire.  |
