Team:UT Dallas/week10

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(Difference between revisions)
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<h2>Week 10</h2>
<h2>Week 10</h2>
<table>
<table>
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<tr><td valign='top'>9/11/11</td><td valign='top'>Incubation: ToxR-FGFR, ctx-CheZ*, ctx-CheZ, ToxR, FGFR, CheY, CheZ,  
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<tr><td valign='top'>9/11/11</td><td valign='top'>Incubation: ToxR-FGFR, ctx-CheZ*, ctx-CheZ, ToxR, FGFR, CheY, CheZ,
</td></tr><tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
</td></tr><tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
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9/12/11 Glycerol Stock: ToxR-FGFR, ctx-CheZ*, ctx-CheZ, ToxR, FGFR, CheY, CheZ,<br>
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<tr><td valign='top'>9/12/11</td><td valign='top'>Glycerol Stock: ToxR-FGFR, ctx-CheZ*, ctx-CheZ, ToxR, FGFR, CheY, CheZ,<br>
Test Digestion/Gel Electrophoresis: FGFR-ToxR, ctx-CheZ-RBS-GFP<br>
Test Digestion/Gel Electrophoresis: FGFR-ToxR, ctx-CheZ-RBS-GFP<br>
Full Digestion/Gel Electrophoresis: FGFR-ToxR, ctx-CheZ-RBS-GFP<br>
Full Digestion/Gel Electrophoresis: FGFR-ToxR, ctx-CheZ-RBS-GFP<br>

Revision as of 23:39, 27 September 2011

Week 10

9/11/11Incubation: ToxR-FGFR, ctx-CheZ*, ctx-CheZ, ToxR, FGFR, CheY, CheZ,
  
9/12/11Glycerol Stock: ToxR-FGFR, ctx-CheZ*, ctx-CheZ, ToxR, FGFR, CheY, CheZ,

Test Digestion/Gel Electrophoresis: FGFR-ToxR, ctx-CheZ-RBS-GFP
Full Digestion/Gel Electrophoresis: FGFR-ToxR, ctx-CheZ-RBS-GFP
Transformation: ToxR'MalE + ctx-CheZ-RBS-GFP, ToxR'MalE/ZIP + ctx-CheZ-RBS-GFP, ToxR'MalE/Zip L267S + ctx-CheZ-RBS-GFP
Plating: ToxR'MalE/ZIP + ctx-CheZ-RBS-GFP
Ligation: scp, Pyear, TetR, ToxR-FGFR, ctx
Incubation: CheZ* (Chlora)

  
9/13/11Transform of PyeaR-ToxR-FGFR, TetR-ToxR-FGFR, scp-ToxR-FGFR

Full Digestion: ToxR-FGFR (at E/X as vector)
Ligation: Vector-> ToxR-FGFR Insert-> ctx
Incubation: CheY

  
9/14/11Transformation: ctx-ToxR-FGFR

Test Digestion: ToxR'MalE, ToxR'MalE/ZIP, ToxR'MalE/Zip L267S
Incubation: scp-ToxR-FGFR, TetR-ToxR-FGFR, PyeaR-ToxR-FGFR (Carb)
Miniprep: CheY
PCR: CheY
Incubation: ToxR'MalE, ToxR'MalE/ZIP, CheZ*

  
9/15/11PCR: CheY

Full Digestion: CheY (X/S), vector pSB1C3
Miniprep/Glycerol Stock: pYeaR-ToxR-FGFR, scp-ToxR-FGFR, ToxR'MalE, ToxR'MalE/ZIP
Test Digestion: pYeaR-ToxR-FGFR, scp-ToxR-FGFR, ToxR'MalE, ToxR'MalE/ZIP
Dephosphorylation: Vector pSB1C3
Ligation: CheY + pSB1c3
Full Digestion/Gel Elctrophoresis/Purification: pYeaR-ToxR-FGFR, scp-ToxR-FGFR, ToxR'MalE, ToxR'MalE/ZIP, CheZ, CheZ*, ctx-CheZ-RBS-GFP, ctx
Ligation: ctx + CheZ*
Transformation: pSB1C3 Vector, pYeaR-ToxR-FGFR, ctx-CheZ-RBS-GFP, CheZ, ctx-GFP, ctx, Chey

  
9/16/11Digestion: ctx-CheZ

Incubation: ToxR'MalE/ZIP, ToxR'MalE,
Ligation: ctx + CheY (retry)

  
9/17/11Incubation: pYeaR-ToxR-FGFR, ctx-CheZ*, CheY
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