Team:UT Dallas/week10

Week 10

9/6/11	Incubation: Germany Parts=[pHKToxR'MalE, pHKToxR'MalE/ZIP, pHKToxR'REI-T39K, pHKToxR'MalE/ZIP L267S, pHKToxR'HisJGCN] (FHK12) CheY, PyeaR-GFP-RBS-TetR-term, scp-GFP-RBS-TetR-Term

9/7/11	Glycerol Stock/Miniprep: [pHKToxR'MalE, pHKToxR'MalE/ZIP, pHKToxR'REI-T39K, pHKToxR'MalE/ZIP L267S, pHKToxR'HisJGCN] Incubation: pYeaR-GFP-RBS-TetR-Term, scp-GFP-RBS-Tetr-Term, AHL inducible Colicin, Pcst-RBS-LuxI-Term Full Digestion/Gel Electrophoresis+Purification: FGFR Ligation 10 Min: Vectors: FGFR, Inserts; ToxR Transformation: ToxR-FGFR

9/8/11	Dilution of Pcst-RBS-:uxI-Term, AHL induible colicin Incubation: DATA COLLECTION=GFP quantification [(a) Psct-RBS-LuxI-Term vs. (b) AHL inducible colicin](DH5-alpha) Glycerol Stock/Miniprep: Pyear-GFP-RBS-TetR-Term, scp-GFP-RBS-TetR-Term Test Digestion: Pyear-GFP-RBS-Term, scp-GFP-RBS-TetR-Term Colony PCR/ Gel Ectrophoresis: Toxr-FGFR

9/9/11	Full Digestion: FGFR, RBS-GFP-TetR-Term, CheY, CheZ, ctx-CheZ Test Digestion: Pyear-GFp-RBS-TetR-Term, scp-GFP-RBS-TetR-Term Ligation 10 Min: Vectors: CheY, CheZ, FGFR, RBS-GFP Inserts: Ctx Transformation: FGFR, ToxR-FGFR, ctx-CheZ-RBS-GFP, CheZ, ctx-CheZ*

9/10/11	Colony PCR: ToxR-FGFR, ctx-CheZ*, ctx-CheZ