Team:UT Dallas/week10

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Revision as of 22:53, 27 September 2011

Week 10

9/12/11 Glycerol Stock: ToxR-FGFR, ctx-CheZ*, ctx-CheZ, ToxR, FGFR, CheY, CheZ,
Test Digestion/Gel Electrophoresis: FGFR-ToxR, ctx-CheZ-RBS-GFP
Full Digestion/Gel Electrophoresis: FGFR-ToxR, ctx-CheZ-RBS-GFP
Transformation: ToxR'MalE + ctx-CheZ-RBS-GFP, ToxR'MalE/ZIP + ctx-CheZ-RBS-GFP, ToxR'MalE/Zip L267S + ctx-CheZ-RBS-GFP
Plating: ToxR'MalE/ZIP + ctx-CheZ-RBS-GFP
Ligation: scp, Pyear, TetR, ToxR-FGFR, ctx
Incubation: CheZ* (Chlora)

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9/11/11	Incubation: ToxR-FGFR, ctx-CheZ*, ctx-CheZ, ToxR, FGFR, CheY, CheZ,


9/13/11	Transform of PyeaR-ToxR-FGFR, TetR-ToxR-FGFR, scp-ToxR-FGFR Full Digestion: ToxR-FGFR (at E/X as vector) Ligation: Vector-> ToxR-FGFR Insert-> ctx Incubation: CheY

9/14/11	Transformation: ctx-ToxR-FGFR Test Digestion: ToxR'MalE, ToxR'MalE/ZIP, ToxR'MalE/Zip L267S Incubation: scp-ToxR-FGFR, TetR-ToxR-FGFR, PyeaR-ToxR-FGFR (Carb) Miniprep: CheY PCR: CheY Incubation: ToxR'MalE, ToxR'MalE/ZIP, CheZ*

9/15/11	PCR: CheY Full Digestion: CheY (X/S), vector pSB1C3 Miniprep/Glycerol Stock: pYeaR-ToxR-FGFR, scp-ToxR-FGFR, ToxR'MalE, ToxR'MalE/ZIP Test Digestion: pYeaR-ToxR-FGFR, scp-ToxR-FGFR, ToxR'MalE, ToxR'MalE/ZIP Dephosphorylation: Vector pSB1C3 Ligation: CheY + pSB1c3 Full Digestion/Gel Elctrophoresis/Purification: pYeaR-ToxR-FGFR, scp-ToxR-FGFR, ToxR'MalE, ToxR'MalE/ZIP, CheZ, CheZ, ctx-CheZ-RBS-GFP, ctx Ligation: ctx + CheZ Transformation: pSB1C3 Vector, pYeaR-ToxR-FGFR, ctx-CheZ-RBS-GFP, CheZ, ctx-GFP, ctx, Chey

9/16/11	Digestion: ctx-CheZ Incubation: ToxR'MalE/ZIP, ToxR'MalE, Ligation: ctx + CheY (retry)

9/17/11	Incubation: pYeaR-ToxR-FGFR, ctx-CheZ*, CheY