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Revision as of 22:42, 27 September 2011 (view source) NathanDavis (Talk | contribs) m (Automatic page editing) ← Older edit		Latest revision as of 22:47, 27 September 2011 (view source) NathanDavis (Talk | contribs) m (Automatic page editing)
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		+	== PCR Amplification of OmpA ==
	'''Project:''' Surface Display		'''Project:''' Surface Display
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	<nowiki>-38 uL ddH20</nowiki>		<nowiki>-38 uL ddH20</nowiki>
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	{{Team:Minnesota/Bottom}}		{{Team:Minnesota/Bottom}}

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Latest revision as of 22:47, 27 September 2011

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PCR Amplification of OmpA

Project: Surface Display

Date: Thu, 14 Apr 2011 00:00:00 GMT

Author: Sukdong Lee (leex4462)

Access: Public

Revision History:

• Mon, 22 Aug 2011 09:13:32 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)

Purpose:

To replicate the ompA gene and place into a pucBB plasmid.

Procedures:

Followed standard protocol.

For 50 ul reaction add:

-1 uL Template

-1 uL of each primer

-5 uL 10x Thermobuffer

-1 uL 10mM ddNTPs

-2.5 uL 10mM MgSO4

-0.5 uL DNA polymerase:Vent

-38 uL ddH20

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