Team:Minnesota/davis950-08102011145942-15
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{{Team:Minnesota/Top}} | {{Team:Minnesota/Top}} | ||
- | <br>== Transformation of pucBB-pBad-ho1, pucBB-pBad-pcyA, and pucBB-pTet-Cph8 == | + | <br> |
+ | == Transformation of pucBB-pBad-ho1, pucBB-pBad-pcyA, and pucBB-pTet-Cph8 == | ||
'''Project:''' Regulatory | '''Project:''' Regulatory | ||
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<!-- Technology built on the Itenso Rtf Converter Framework --> | <!-- Technology built on the Itenso Rtf Converter Framework --> | ||
<nowiki>Transformed electrocompetent cells with ligation products of pucBB-pBad-ho1, pucBB-pBad-pcyA, and pucBB-pTet-Cph8. Added 5 ul of each ligation product to 100 ul compentent cells. ho1 ligation product contained excessive salt and caused arc during electroporation. Attempted a second electroporation with 2.5 ul ho1 ligation product which was successful. Plates placed in 37 degree C incubator in Claudia's lab. David Babson will remove plates first thing in the morning and will place them in cooler. NOTE per David: do NOT pick colonies which turn red on the plate.</nowiki> | <nowiki>Transformed electrocompetent cells with ligation products of pucBB-pBad-ho1, pucBB-pBad-pcyA, and pucBB-pTet-Cph8. Added 5 ul of each ligation product to 100 ul compentent cells. ho1 ligation product contained excessive salt and caused arc during electroporation. Attempted a second electroporation with 2.5 ul ho1 ligation product which was successful. Plates placed in 37 degree C incubator in Claudia's lab. David Babson will remove plates first thing in the morning and will place them in cooler. NOTE per David: do NOT pick colonies which turn red on the plate.</nowiki> | ||
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{{Team:Minnesota/Bottom}} | {{Team:Minnesota/Bottom}} |
Latest revision as of 22:46, 27 September 2011
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