"
Team:Washington/Magnetosomes/GibsonResults
From 2011.igem.org
| Line 14: | Line 14: | ||
[[File:Washington_iGEM2011_pSB.png|thumb|right|500px|pSB vector plate]] | [[File:Washington_iGEM2011_pSB.png|thumb|right|500px|pSB vector plate]] | ||
| - | Once the Gibson efficiences were calculated for each set of plates, the average Gibson efficiency was calculated. The average Gibson efficiency of using our pGA vectors was '''0.991129032'''. This value is significantly higher than that of the pSB vectors (efficiency = 0.116395664). | + | Once the Gibson efficiences were calculated for each set of plates, the average Gibson efficiency was calculated. The average Gibson efficiency of using our pGA vectors was '''0.991129032'''. This value is significantly higher than that of the pSB vectors (efficiency = 0.116395664). Visually, one can see that there are more fluorescent colonies on the pGA plate vs the pSB plate. |
[[File:Washington_iGEM2011_VectorComparison.png|thumb|center|500px|Gibson Assembly Efficiency Comparison]] | [[File:Washington_iGEM2011_VectorComparison.png|thumb|center|500px|Gibson Assembly Efficiency Comparison]] | ||
Based on the comparison presented, we can be certain that our Gibson Assembly vectors (pGA vectors) are a great choice for gene assemblies! | Based on the comparison presented, we can be certain that our Gibson Assembly vectors (pGA vectors) are a great choice for gene assemblies! | ||
Revision as of 22:26, 27 September 2011
Comparison between pGA and pSB vectors
To quantitatively illustrate that better cloning efficiency is achieved by using pGA vectors vs standard pSB vectors, a pGA vector assay was conducted. The Gibson products made using our pGA vectors and the standard pSB vectors were plated separately over 6 plates, and the Gibson efficiency was calculated for each plate.
Efficiency was calculated by dividing the # of bright colonies by the # of total colonies - # of background colonies. Gibson Efficiency = Bright colonies/Total - Background)
Once the Gibson efficiences were calculated for each set of plates, the average Gibson efficiency was calculated. The average Gibson efficiency of using our pGA vectors was 0.991129032. This value is significantly higher than that of the pSB vectors (efficiency = 0.116395664). Visually, one can see that there are more fluorescent colonies on the pGA plate vs the pSB plate.
Based on the comparison presented, we can be certain that our Gibson Assembly vectors (pGA vectors) are a great choice for gene assemblies!
