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Team:Washington/Protocols/pGA
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Revision as of 21:29, 27 September 2011
pGA vector Assay
- Prepare this mixture on ice to prevent the reaction from beginning early
- Add 15uL of Gibson MasterMix to each tube.
- Add ~20-50 ng of purified Backbone DNA to the reaction tube.
- Add ~20-50 ng of purified Insert DNA to the reaction tube.
- Fill the remaining tubes with ice cold, distilled H20 to ensure a final volume of 20 uL
- After making sure all tubes are appropriately labeled, incubate all samples for ~1 hour @ 50oC.
