Team:UCSF/MGFP5

From 2011.igem.org

(Difference between revisions)
(Created page with "{{Template:UCSF}}")
Line 1: Line 1:
{{Template:UCSF}}
{{Template:UCSF}}
 +
 +
<html>
 +
</head>
 +
 +
 +
 +
 +
 +
<link rel="stylesheet" type="text/css" href="http://tinyurl.com/twilightsparkles"/>
 +
 +
 +
 +
<style>
 +
#rightcontent {width:755px; float:left; background-color: #F5F5F5; margin-left: 8px;  margin-top:10px;}
 +
 +
</style>
 +
 +
</head>
 +
 +
<div id="rightcontenttext"> <p><p>
 +
<h3red>MgFP5</h3red> <p>
 +
 +
<regulartext>Mgfp-5 is an adhesive protein present in the foot of mussels. It is known to be an
 +
important part of what makes mussels stick on surfaces. We hoped to express
 +
mgfp-5 on the surface of S. cerevisiae to enhance its adherence to materials such as
 +
polystyrene or treated plastics and promote adherence to other yeast cells.
 +
 +
We discovered that it already was a part in the Parts Registry, but only
 +
characterized in E. coli. The previous team expressed it in E. coli and ran a protein
 +
quantitative assay, the results of which can be found <a href=http://partsregistry.org/Part:BBa_K197018>here</a>. We decided that we’d take a different approach in assaying the
 +
results, to observe what mgfp-5-expressing yeast grew like under the microscope
 +
and how it adhered in flowing conditions.
 +
 +
Our results show conclusive evidence that mgfp-5-expressing yeast “cluster”, form
 +
clumps of cells that grow on and around on another, more often and to a larger
 +
degree than non-mgfp-5-expressing yeast. We hope that this could be used to create
 +
a biomaterial or a bioadhesive that could be natural and biodegradable.</regulartext>
 +
 +
 +
</div>
 +
 +
 +
 +
</html>

Revision as of 05:47, 27 September 2011

introduction
the team
our project
parts
requisites
attributions
extras

MgFP5

Mgfp-5 is an adhesive protein present in the foot of mussels. It is known to be an important part of what makes mussels stick on surfaces. We hoped to express mgfp-5 on the surface of S. cerevisiae to enhance its adherence to materials such as polystyrene or treated plastics and promote adherence to other yeast cells. We discovered that it already was a part in the Parts Registry, but only characterized in E. coli. The previous team expressed it in E. coli and ran a protein quantitative assay, the results of which can be found here. We decided that we’d take a different approach in assaying the results, to observe what mgfp-5-expressing yeast grew like under the microscope and how it adhered in flowing conditions. Our results show conclusive evidence that mgfp-5-expressing yeast “cluster”, form clumps of cells that grow on and around on another, more often and to a larger degree than non-mgfp-5-expressing yeast. We hope that this could be used to create a biomaterial or a bioadhesive that could be natural and biodegradable.

Retrieved from "http://2011.igem.org/Team:UCSF/MGFP5"