"
Team:Harvard/Lambda Red
From 2011.igem.org
(Difference between revisions)
(→Lambda Red) |
(→Lambda Red) |
||
| Line 3: | Line 3: | ||
<div class="whitebox"> | <div class="whitebox"> | ||
== Lambda Red == | == Lambda Red == | ||
| - | Lambda red recombineering makes use of homologous recombination systems to allow the insertion of constructs into the genome. It is accomplished in two steps, as shown in the diagram below and in our [https://2011.igem.org/Team:Harvard/Protocols Protocols] section. | + | Lambda red recombineering makes use of homologous recombination systems to allow the insertion of constructs into the genome. It is accomplished in two steps, as shown in the diagram below and in our [https://2011.igem.org/Team:Harvard/Protocols Protocols] section. Also see [https://2011.igem.org/Team:Harvard/Results/Lambda_Red Lambda Red results] for how we used lambda red to build our selection system. |
</div> | </div> | ||
<div class="whitebox"> | <div class="whitebox"> | ||
Revision as of 04:05, 26 September 2011
Overview | MAGE | Chip-Based Synthesis | Lambda Red | Protocols
Lambda Red
Lambda red recombineering makes use of homologous recombination systems to allow the insertion of constructs into the genome. It is accomplished in two steps, as shown in the diagram below and in our Protocols section. Also see Lambda Red results for how we used lambda red to build our selection system.
Figure 1. Lambda Red, PCR to get the required insertion product (zeocin in this example) 915px
