Revision as of 03:23, 26 September 2011

Make It or Break It: Diesel Production and Gluten Destruction, the Synthetic Biology Way

Synthetic biology holds great promise regarding the production of important compounds, and the degradation of harmful ones. This summer, we harnessed the power of synthetic biology to meet the world’s needs for fuel and medicine.

Make It: Diesel Production We constructed a strain of Escherichia coli that produces a variety of alkanes, the main constituents of diesel fuel, by introducing a pair of genes recently shown convert fatty acid synthesis intermediates into alkanes.

Break It: Gluten Destruction We identified a protease with gluten-degradation potential, and then reengineered it to have greatly increased gluten-degrading activity, allowing for the breakdown of gluten in the digestive track when taken in pill form.

iGEM Toolkits To enable next-generation cloning of standard biological parts, we built BioBrick vectors optimized for Gibson assembly and used them to bring to the Parts Registry the Magnetosome Toolkit: a set of genes for biofabrication of magnetic particles.

File:Gibson Assembly and Magnetosomes for Wiki.png

University of Washington Anaspec

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+=<center>Make It or Break It: Diesel Production and Gluten Destruction, the Synthetic Biology Way</center>=
+Synthetic biology holds great promise regarding the production of important compounds, and the degradation of harmful ones. This summer, we harnessed the power of synthetic biology to meet the world’s needs for fuel and medicine.
-=<center>'''Make It or Break It: Diesel Production and Gluten Destruction, the Synthetic Biology Way'''</center>=
+[https://2011.igem.org/Team:Washington/Alkanes/Background '''Make It: Diesel Production''']  We constructed a strain of ''Escherichia coli'' that produces a variety of alkanes, the main constituents of diesel fuel, by introducing a pair of genes recently shown convert fatty acid synthesis intermediates into alkanes.
-<br/>
-<center><gallery widths="250px" heights="300px" perrow="3">
+[https://2011.igem.org/Team:Washington/Celiacs/Background '''Break It: Gluten Destruction''']  We identified a protease with gluten-degradation potential, and then reengineered it to have greatly increased gluten-degrading activity, allowing for the breakdown of gluten in the digestive track when taken in pill form.
-Image:Diesel Production for Wiki.png|'''Make It:  Diesel Production'''
-Image:Gluten Destruction for Wiki.png|'''Break It:  Gluten Destruction'''
-Image:Gibson Assembly and Magnetosomes for Wiki.png|'''The GibsonBricks and Magnetosome ToolKit'''<br>
-</gallery></center>
+[https://2011.igem.org/Team:Washington/Magnetosomes/Background '''iGEM Toolkits''']  To enable next-generation cloning of standard biological parts, we built BioBrick vectors optimized for Gibson assembly and used them to bring to the Parts Registry the Magnetosome Toolkit: a set of genes for biofabrication of magnetic particles.
-<br/>
-Synthetic biology holds great promise regarding the production of important compounds, and the degradation of harmful ones. This summer, we harnessed the power of synthetic biology to meet the world’s needs for fuel and medicine.
-[https://2011.igem.org/Team:Washington/Alkanes/Background '''Make It: Diesel Production''']  We constructed a strain of ''Escherichia coli'' that produces a variety of alkanes, the main constituents of diesel fuel, by introducing a pair of genes recently shown convert fatty acid synthesis intermediates into alkanes.
+[[File:Washington_Spacer.jpg|35px]]
+[[Image:Diesel Production for Wiki.png|280px|link=https://2011.igem.org/Team:Washington/Alkanes/Background]]
+[[File:Washington_Spacer.jpg|35px]]
+[[Image:Gluten Destruction for Wiki.png|280px|link=https://2011.igem.org/Team:Washington/Celiacs/Background]]
+[[File:Washington_Spacer.jpg|35px]]
+[[Image:Gibson Assembly and Magnetosomes for Wiki.png|280px|link=https://2011.igem.org/Team:Washington/Magnetosomes/Background]]
+[[File:Washington_Spacer.jpg|35px]]
-[https://2011.igem.org/Team:Washington/Celiacs/Background '''Break It: Gluten Destruction''']  We identified a protease with gluten-degradation potential, and then reengineered it to have greatly increased gluten-degrading activity, allowing for the breakdown of gluten in the digestive track when taken in pill form.
-[https://2011.igem.org/Team:Washington/Magnetosomes/Background '''iGEM Toolkits''']  To enable next-generation cloning of standard biological parts, we built BioBrick vectors optimized for Gibson assembly and used them to bring to the Parts Registry the Magnetosome Toolkit: a set of genes for biofabrication of magnetic particles.