Team:Harvard/Results/Chip Synthesis

From 2011.igem.org

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[[Team:Harvard/Results | Overview]] | [[Team:Harvard/Results/MAGE | MAGE]] | [[Team:Harvard/Results/Lambda_Red | Lambda Red]]| [[Team:Harvard/Results/Chip_Synthesis | Chip-Based Library]] | [[Team:Harvard/Results/One-Hybrid_Selection | One-Hybrid Selection]] | [[Team:Harvard/Results/Zinc_Finger_Binders | Zinc Finger Binders]] | [[Team:Harvard/Results/Biobricks | Biobricks]]
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Revision as of 00:43, 26 September 2011

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Overview | Biobricks | Source Code | Acknowledgments | Accomplishments

qPCR curves

When performing a qPCR, one wants to run the reaction while the growth is still exponential. During this phase, all the oligos are replicated at equal rates. Once growth levels off, the reaction should be stopped as the oligos are now being replicated unequally. Each of the graphs below represents a qPCR for one of our sub-pools.

CB Top
CB Bot
FH Top
FH Bot
Myc 198
Myc 981

Probability data for the 783 fingers that bind to GNN triplets. Note the high probability of leucine at position 4 and arginine at position 6.

























Sequencing results of Library transformation

Error rates

Still being updated

  • Good: 57.8% (22/38)
  • Single SNP: 5.3% (2/38)
  • Multiple SNPs: 18.4% (7/38)
  • Frame shift: 18.4% (7/38)

Distributions

Still being updated Each of the sequenced zinc fingers have original F1 fingers.

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