qPCR curves

When performing a qPCR, one wants to run the reaction while the growth is still exponential. During this phase, all the oligos are replicated at equal rates. Once growth levels off, the reaction should be stopped as the oligos are now being replicated unequally. Each of the graphs below represents a qPCR for one of our sub-pools.

Probability data for the 783 fingers that bind to GNN triplets. Note the high probability of leucine at position 4 and arginine at position 6.

Probability data for the 128 fingers that bind to TNN triplets. Note the high probability of leucine at position 4.

Probability data for the 16 fingers that bind to CNN triplets. There may not be enough data to consider this information statistically significant

Probability data for the 29 fingers that bind to ANN triplets. There may not be enough data to consider this information statistically significant

Probability data for the 298 fingers that bind to NGN triplets. The position 4 leucine motif remains. There is also a high probability (> 0.5) of a histidine at position 3 and an arginine at position 6.

Probability data for the 177 fingers that bind to NTN triplets. The position 4 leucine motif remains.