Team:Harvard/Results/Chip Synthesis

From 2011.igem.org

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Revision as of 15:32, 25 September 2011

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Overview | MAGE | Lambda Red| Chip-Based Library | One-Hybrid Selection | Zinc Finger Binders | Biobricks


qPCR curves

CB Top
CB Bot
FH Top
FH Bot
Myc 198
Myc 981

Probability data for the 783 fingers that bind to GNN triplets. Note the high probability of leucine at position 4 and arginine at position 6.

























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Sequencing results of Library transformation

Error rates

Still being updated

  • Good: 57.8% (22/38)
  • Single SNP: 5.3% (2/38)
  • Multiple SNPs: 18.4% (7/38)
  • Frame shift: 18.4% (7/38)

Distributions

Still being updated Each of the sequenced zinc fingers have original F1 fingers.

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