Team:USC/Notebook/Week7

Brainstorming

Week 1

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 13-14

Week 7:

07/19/2011
1. Gel verification for CASO (1-8)
Observation: in second row, GFP spcer, tetR spacer, CRISPR#1, and CRISPR #2
2. Gel verification by digestion
3. Purify tetR from Gel
4. Ligate pSB1C3 with tetR, place in the 15c incubator overnight
5. Transformation

07/21/2011
1. Transform ligations of tetR in DH5α and top10 competent cells
2. Inoculate PCOLA, PCDF and CRISPR

07/22/2011
1. Mini-prep for :
tetR in DH5α and top10 (16 samples)
CRISPR (2 samples)
PCOLA (1 sample)
PCDF (5 samples)
2. Digest tetR with EcoR1 and Pst1
3. Test the digested tetR by running a gel