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Team:WashU/Notebook/September2011
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| + | ==Sept. 24== | ||
| + | pSB1C3 plasmid arrived; genes ligated into plasmid by MolBio group | ||
| + | Transformed E. coli | ||
| + | LB plates: 150 mL h20 + 6.25 g LB broth + 3.75 g Bacto Agar to Erlenmeyer flask | ||
| + | add water to 250 mL (final volume ended up being ~130 mL) | ||
| + | |||
| + | Chloramphenicol stock solution: 0.07 g chloramphenicol into 2 mL, filter-sterilize; add ~130 uL stock solution into LB (1:1000 dilution) | ||
Revision as of 01:52, 25 September 2011
Sept. 24
pSB1C3 plasmid arrived; genes ligated into plasmid by MolBio group Transformed E. coli LB plates: 150 mL h20 + 6.25 g LB broth + 3.75 g Bacto Agar to Erlenmeyer flask add water to 250 mL (final volume ended up being ~130 mL)
Chloramphenicol stock solution: 0.07 g chloramphenicol into 2 mL, filter-sterilize; add ~130 uL stock solution into LB (1:1000 dilution)
