Revision as of 02:10, 24 September 2011

PROJECT

RESULTS

CONSIDERATIONS

ABOUT US

NOTEBOOK

ATTRIBUTIONS

File:Protocols img1.gif

Cloning

|| Transformation || Overnight || Miniprep || 3A Assembly ||
|| PCR Amplification || PCR Purification ||

File:Protocols img22.jpg

Primers and Design

|| Primer Design || Oligo Annealing || DNA Sequencing ||
|| Linearized Plasmid Backbones ||

Cells and Solutions

|| Top10 Chemically Competent Cells || CCMB80 Buffer ||
|| Media || Glycerol Stocks || Agarose Gels || Plates ||
|| Antibiotic Stocks ||

@@ Line 4: / Line 4: @@
 <!-- ----------------------Cloning------------------------ -->
-<div style="background:#666666;height:148px;width:740px;border-radius: 2px;">
+<div style="background:#777777;height:148px;width:740px;border-radius: 2px;">
 <DIV id="valerie" style="position:right;margin: 0px 0px 0px 0px; padding:10px;position:relative;" allowtransparency="true">
 [[Image: Protocols_img1.gif|left|200px|]]
@@ Line 10: / Line 10: @@
 <div style="margin:10px 0px 0px 150px;color:#ffffff;font:28px helvetica;font-weight:25;">
 &nbsp;'''Cloning''' <br /></div><div style="margin:5px 0px 0px 240px;color:#ffffff">
-|| [[Team:Northwestern/Notebook/Protocols/Transformation| Transformation]] ||[[Team:Northwestern/Notebook/Protocols/Overnight| Overnight]] ||
+|| [[Team:Northwestern/Notebook/Protocols/Transformation| <span style="color:#000000;">Transformation</span>]] ||[[Team:Northwestern/Notebook/Protocols/Overnight|<span style="color:#000000;"> Overnight</span>]] ||
-[[Team:Northwestern/Notebook/Protocols/Miniprep| Miniprep]] ||
+[[Team:Northwestern/Notebook/Protocols/Miniprep|<span style="color:#000000;"> Miniprep</span>]] ||
-[[Team:Northwestern/Notebook/Protocols/3A_Assembly| 3A Assembly]] ||<br />
+[[Team:Northwestern/Notebook/Protocols/3A_Assembly|<span style="color:#000000;"> 3A Assembly</span>]] ||<br />
-|| [[Team:Northwestern/Notebook/Protocols/PCR_Amplification| PCR Amplification]] ||
+|| [[Team:Northwestern/Notebook/Protocols/PCR_Amplification|<span style="color:#000000;"> PCR Amplification</span>]] ||
-[[Team:Northwestern/Notebook/Protocols/PCR_Purification| PCR Purification]] ||
+[[Team:Northwestern/Notebook/Protocols/PCR_Purification|<span style="color:#000000;"> PCR Purification</span>]] ||
 </div>
 </div>
@@ Line 21: / Line 21: @@
 <!-- ----------------------Primers and Construction------------------------ -->
-<div style="background:#666666;height:148px;width:740px;border-radius: 2px;">
+<div style="background:#777777;height:148px;width:740px;border-radius: 2px;">
 <DIV id="valerie" style="position:right;margin: 0px 0px 0px 0px; padding:10px;position:relative;" allowtransparency="true">
 [[Image: Protocols_img22.jpg|left|200px|]]
@@ Line 27: / Line 27: @@
 <div style="margin:10px 0px 0px 150px;color:#ffffff;font:28px helvetica;font-weight:25;">
 &nbsp;'''Primers and Design''' <br /></div><div style="margin:5px 0px 0px 240px;color:#ffffff">
-|| [[Team:Northwestern/Notebook/Protocols/Primer_Design| Primer Design]] ||
+|| [[Team:Northwestern/Notebook/Protocols/Primer_Design| <span style="color:#000000;"> Primer Design</span>]] ||
-[[Team:Northwestern/Notebook/Protocols/Oligo_Annealing| Oligo Annealing]] ||
+[[Team:Northwestern/Notebook/Protocols/Oligo_Annealing| <span style="color:#000000;"> Oligo Annealing</span>]] ||
-[[Team:Northwestern/Notebook/Protocols/Sequencing| DNA Sequencing]] ||<br />
+[[Team:Northwestern/Notebook/Protocols/Sequencing| <span style="color:#000000;"> DNA Sequencing</span>]] ||<br />
-|| [[Team:Northwestern/Notebook/Protocols/Linearized_Plasmid_Backbones| Linearized Plasmid Backbones]] ||
+|| [[Team:Northwestern/Notebook/Protocols/Linearized_Plasmid_Backbones| <span style="color:#000000;"> Linearized Plasmid Backbones</span>]] ||
 </div>
 </div>
@@ Line 38: / Line 38: @@
 <!-- ----------------------Cells and Solutions------------------------ -->
-<div style="background:#666666;height:148px;width:740px;border-radius: 2px;">
+<div style="background:#777777;height:148px;width:740px;border-radius: 2px;">
 <DIV id="valerie" style="position:right;margin: 0px 0px 0px 0px; padding:10px;position:relative;" allowtransparency="true">
 [[Image: Protocols_img33.jpg|left|200px]]
@@ Line 44: / Line 44: @@
 <div style="margin:10px 0px 0px 150px;color:#ffffff;font:28px helvetica;font-weight:25;">
 &nbsp;'''Cells and Solutions''' <br /></div><div style="margin:5px 0px 0px 240px;color:#ffffff">
-|| [[Team:Northwestern/Notebook/Protocols/Top10_Competent| Top10 Chemically Competent Cells]] ||
+|| [[Team:Northwestern/Notebook/Protocols/Top10_Competent|  <span style="color:#000000;">Top10 Chemically Competent Cells</span>]] ||
-[[Team:Northwestern/Notebook/Protocols/CCMB80| CCMB80 Buffer]] ||<br />
+[[Team:Northwestern/Notebook/Protocols/CCMB80|  <span style="color:#000000;">CCMB80 Buffer</span>]] ||<br />
-|| [[Team:Northwestern/Notebook/Protocols/Media| Media]] ||
+|| [[Team:Northwestern/Notebook/Protocols/Media|  <span style="color:#000000;">Media</span>]] ||
-[[Team:Northwestern/Notebook/Protocols/Glycerol| Glycerol Stocks]] ||
+[[Team:Northwestern/Notebook/Protocols/Glycerol|  <span style="color:#000000;">Glycerol Stocks</span>]] ||
-[[Team:Northwestern/Notebook/Protocols/Agarose_Gels| Agarose Gels]] ||
+[[Team:Northwestern/Notebook/Protocols/Agarose_Gels|  <span style="color:#000000;">Agarose Gels</span>]] ||
-[[Team:Northwestern/Notebook/Protocols/Plates| Plates]] ||<br />
+[[Team:Northwestern/Notebook/Protocols/Plates|  <span style="color:#000000;">Plates</span>]] ||<br />
-||[[Team:Northwestern/Notebook/Protocols/Antibiotic_Stocks| Antibiotic Stocks]] ||
+||[[Team:Northwestern/Notebook/Protocols/Antibiotic_Stocks|  <span style="color:#000000;">Antibiotic Stocks</span>]] ||
 </div>
 </div>
 <!-- ----------------------------------------------------------- -->