Team:Washington/Magnetosomes/Parts

From 2011.igem.org

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=='''The Gibson Assembly Toolkit'''==
=='''The Gibson Assembly Toolkit'''==
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590010 BBa_K590010 '''pGA1A3_pLacGFP''']
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This is a high copy plasmid backbone which has ampicillin resistance, with pLac promoter and GFP.
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590011 BBa_K590011 '''pGA1C3_pLacGFP''']
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This is a high copy plasmid backbone which has Chloramphenicol resistance, with pLac promoter and GFP.
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590012 BBa_K590012 '''pGA4C5_pLacGFP''']
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This is a low copy plasmid backbone which has Chloramphenicol resistance, with pLac promoter and GFP.
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590013 BBa_K590013 '''pGA4A5_pLacGFP''']
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This is a low copy plasmid backbone which has Ampicillin resistance, with pLac promoter and GFP .
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590014 BBa_K590014 '''pGA3K3_pLacGFP''']
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This is a medium copy plasmid backbone which has Kanamycin resistance, with pLac promoter and GFP.

Revision as of 02:39, 23 September 2011


iGEM Toolkits: Parts Submitted


iGem Toolkits: submitted a total twenty-two parts to the registry:

  • 10 magnetosome gene-groups,
  • 2 mam gene-sfGFP fusions,
  • 3 parts of the essential magnetosome gene- assembly, and
  • 5 pGA vectors.

Magnetosome Toolkit Favorite Parts

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590015 BBa_K590015 sfGFP_mamK_pGA1C3]

This part consists of mamK gene from Magnetospirillum magneticum strain AMB-1, sfGFP in the backbone of pGA1C3. mamK was previously reported for its importance proper magnetosome chain organization in magnetic bacteria; it is bacterial actin-like cytoskeleton protein required for proper alignment of the magnetosomes in a chain.

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590016 BBa_K590016 sfGFP_mamI_pGA1C3]

This part consists of mamI gene from Magnetospirillum magneticum strain AMB-1, sfGFP in the backbone of pGA1C3. MamI is a membrane-localized protein that localizes magnetic vesicles to the surface of cells, thus forming characteristic magnetosome chains.

The Gibson Assembly Toolkit

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590010 BBa_K590010 pGA1A3_pLacGFP]

This is a high copy plasmid backbone which has ampicillin resistance, with pLac promoter and GFP.

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590011 BBa_K590011 pGA1C3_pLacGFP]

This is a high copy plasmid backbone which has Chloramphenicol resistance, with pLac promoter and GFP.

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590012 BBa_K590012 pGA4C5_pLacGFP]

This is a low copy plasmid backbone which has Chloramphenicol resistance, with pLac promoter and GFP.

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590013 BBa_K590013 pGA4A5_pLacGFP]

This is a low copy plasmid backbone which has Ampicillin resistance, with pLac promoter and GFP .

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590014 BBa_K590014 pGA3K3_pLacGFP]

This is a medium copy plasmid backbone which has Kanamycin resistance, with pLac promoter and GFP.


Gene AMB Number Cluster Membership Member of 28 genes list? (specific*/related**) Function Summary (Vesicle chain formation, and/or biomineralization) Gene Function
mamH amb0961 mamAB Related
mamI amb0962 mamAB Specific Vesicle, (Chain Formation?) >berkeley 2010: Loss causes no membrane formation, is localized onto chains
mamE amb0963 mamAB; mam Islet Related >Membrane-bound serine protease required for magnetite formation; might control the localization of other magnetosome proteins
mamJ amb0964 mamAB; mam Islet Specific Chain Formation >Proper magnetosome chain organization/assembly
mamK amb0965 mamAB; mam Islet Related Chain Formation >required for proper magnetosome chain organization; *bacterial actin-like cytoskeleton protein required for proper alignment of the magnetosomes in a chain, shown to localize the mamI
mamL amb0966 mamAB; mam Islet Specific Vesicle, biomineralization >berkely 2010: Crucial to mangneosome membrane creation, shown to be spread across the cell membrane and sometimes forms lines
mamM amb0967 mamAB Related >biomineralization, involved in iron transport, magnetite nucleation, or establishement of the proper chemical enviornment for magnetite synthesis in the magnetosome
mamN amb0968 mamAB Related >biomineralization, involved in iron transport, magnetite nucleation, or establishement of the proper chemical enviornment for magnetite synthesis in the magnetosome
mamO amb0969 mamAB Related >biomineralization, involved in iron transport, magnetite nucleation, or establishement of the proper chemical enviornment for magnetite synthesis in the magnetosome
mamP amb0970 mamAB Related Biomineralization >berkeley 2010: loss causes weak magnetic response, with large but fewer crystals
mamA amb0971 mamAB Related >Required for magnetosome activation; activation of vessicles
mamQ amb0972 mamAB; mam Islet Related >ORF; formation/maintenance of magnetosome membranes
mamR amb0973 mamAB Specific Chain formation, Biomineralization >ORF; plays a role in controlling both particle number and size of magnetite cyrstals
mamB amb0974 mamAB Related Vesicle, Biomineralization >indirect role in magnetosome membrane invagination and biomineralization; magnetosome compartment formation
mamS amb0975 mamAB Specific
mamT amb0976 mamAB Specific Biomineralization >magnetite crystal growth; participates in different steps during magnetite synthesis
mamU amb0977 mamAB Related
mamV amb0978 mamAB N/A
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