Team:Washington/Magnetosomes/Parts

From 2011.igem.org

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iGem Toolkits: submitted a total twenty-two parts to the registry: 10 magnetosome gene-groups, 2 mam gene-sfGFP fusions, 3 parts of the essential magnetosome gene- assembly, and 5 pGA vectors.
iGem Toolkits: submitted a total twenty-two parts to the registry: 10 magnetosome gene-groups, 2 mam gene-sfGFP fusions, 3 parts of the essential magnetosome gene- assembly, and 5 pGA vectors.
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{| class="wikitable"
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|-
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! Gene
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! AMB Number
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! Cluster Membership
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! Member of 28 genes list? (specific*/related**)
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! Function Summary (Vesicle chain formation, and/or biomineralization)
 +
! Gene Function
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|-
 +
| mamH
 +
| amb0961
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| mamAB
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| Related
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|
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|
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|-
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| mamI
 +
| amb0962
 +
| mamAB
 +
| Specific
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| Vesicle, (Chain Formation?)
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| >berkeley 2010: Loss causes no membrane formation, is localized onto chains
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|-
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| mamE
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| amb0963
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| mamAB; mam Islet
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| Related
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|
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| >Membrane-bound serine protease required for magnetite formation; might control the localization of other magnetosome proteins
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|-
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| mamJ
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| amb0964
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| mamAB; mam Islet
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| Specific
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| Chain Formation
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| >Proper magnetosome chain organization/assembly
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|-
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| mamK
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| amb0965
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| mamAB; mam Islet
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| Related
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| Chain Formation
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| >required for proper magnetosome chain organization; *bacterial actin-like cytoskeleton protein required for proper alignment of the magnetosomes in a chain, shown to localize the mamI
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|-
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| mamL
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| amb0966
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| mamAB; mam Islet
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| Specific
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| Vesicle, biomineralization
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| >berkely 2010: Crucial to mangneosome membrane creation, shown to be spread across the cell membrane and sometimes forms lines
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|-
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| mamM
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| amb0967
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| mamAB
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| Related
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|
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| >biomineralization, involved in iron transport, magnetite nucleation, or establishement of the proper chemical enviornment for magnetite synthesis in the magnetosome
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|-
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| mamN
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| amb0968
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| mamAB
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| Related
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|
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| >biomineralization, involved in iron transport, magnetite nucleation, or establishement of the proper chemical enviornment for magnetite synthesis in the magnetosome
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|-
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| mamO
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| amb0969
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| mamAB
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| Related
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|
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| >biomineralization, involved in iron transport, magnetite nucleation, or establishement of the proper chemical enviornment for magnetite synthesis in the magnetosome
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|- 
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| mamP
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| amb0970
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| mamAB
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| Related
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| Biomineralization
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| >berkeley 2010: loss causes weak magnetic response, with large but fewer crystals
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|-
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| mamA
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| amb0971
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| mamAB
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| Related
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|
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| >Required for magnetosome activation; activation of vessicles
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|-
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| mamQ
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| amb0972
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| mamAB; mam Islet
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| Related
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|
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| >ORF; formation/maintenance of magnetosome membranes
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|-
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| mamR
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| amb0973
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| mamAB
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| Specific
 +
| Chain formation, Biomineralization
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| >ORF; plays a role in controlling both particle number and size of magnetite cyrstals
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|-
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| mamB
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| amb0974
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| mamAB
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| Related
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| Vesicle, Biomineralization
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| >indirect role in magnetosome membrane invagination and biomineralization; magnetosome compartment formation
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|-
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| mamS
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| amb0975
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| mamAB
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| Specific
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|
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|
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|-
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| mamT
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| amb0976
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| mamAB
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| Specific
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| Biomineralization
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| >magnetite crystal growth; participates in different steps during magnetite synthesis
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|-
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| mamU
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| amb0977
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| mamAB
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| Related
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|
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|
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|-
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| mamV
 +
| amb0978
 +
| mamAB
 +
| N/A
 +
|
 +
|
 +
|-
 +
|}

Revision as of 02:07, 23 September 2011


iGEM Toolkits: Parts Submitted


iGem Toolkits: submitted a total twenty-two parts to the registry: 10 magnetosome gene-groups, 2 mam gene-sfGFP fusions, 3 parts of the essential magnetosome gene- assembly, and 5 pGA vectors.


Gene AMB Number Cluster Membership Member of 28 genes list? (specific*/related**) Function Summary (Vesicle chain formation, and/or biomineralization) Gene Function
mamH amb0961 mamAB Related
mamI amb0962 mamAB Specific Vesicle, (Chain Formation?) >berkeley 2010: Loss causes no membrane formation, is localized onto chains
mamE amb0963 mamAB; mam Islet Related >Membrane-bound serine protease required for magnetite formation; might control the localization of other magnetosome proteins
mamJ amb0964 mamAB; mam Islet Specific Chain Formation >Proper magnetosome chain organization/assembly
mamK amb0965 mamAB; mam Islet Related Chain Formation >required for proper magnetosome chain organization; *bacterial actin-like cytoskeleton protein required for proper alignment of the magnetosomes in a chain, shown to localize the mamI
mamL amb0966 mamAB; mam Islet Specific Vesicle, biomineralization >berkely 2010: Crucial to mangneosome membrane creation, shown to be spread across the cell membrane and sometimes forms lines
mamM amb0967 mamAB Related >biomineralization, involved in iron transport, magnetite nucleation, or establishement of the proper chemical enviornment for magnetite synthesis in the magnetosome
mamN amb0968 mamAB Related >biomineralization, involved in iron transport, magnetite nucleation, or establishement of the proper chemical enviornment for magnetite synthesis in the magnetosome
mamO amb0969 mamAB Related >biomineralization, involved in iron transport, magnetite nucleation, or establishement of the proper chemical enviornment for magnetite synthesis in the magnetosome
mamP amb0970 mamAB Related Biomineralization >berkeley 2010: loss causes weak magnetic response, with large but fewer crystals
mamA amb0971 mamAB Related >Required for magnetosome activation; activation of vessicles
mamQ amb0972 mamAB; mam Islet Related >ORF; formation/maintenance of magnetosome membranes
mamR amb0973 mamAB Specific Chain formation, Biomineralization >ORF; plays a role in controlling both particle number and size of magnetite cyrstals
mamB amb0974 mamAB Related Vesicle, Biomineralization >indirect role in magnetosome membrane invagination and biomineralization; magnetosome compartment formation
mamS amb0975 mamAB Specific
mamT amb0976 mamAB Specific Biomineralization >magnetite crystal growth; participates in different steps during magnetite synthesis
mamU amb0977 mamAB Related
mamV amb0978 mamAB N/A
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