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Team:Washington/Magnetosomes/Background
From 2011.igem.org
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== iGEM Toolkits == | == iGEM Toolkits == | ||
| - | As with the expansion of the iGEM competition, many iGEM teams have started to investigate the possibility | + | As with the expansion of the iGEM competition, many iGEM teams have started to investigate the possibility of working with large-scale genomes. Large-scale gene manipulation often requires the use of tools which allow multiple gene inserts as to bring the cloning project from single gene level to a multiple gene level. However, the current BioBrick standard vectors available through iGEM are not designed for multiple-insert cloning. Therefore, the UW iGEM team decided to research methods to improve cloning efficiency and as a result, two "toolkits" were submitted to the registry. |
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=== Gibson Assembly Toolkit === | === Gibson Assembly Toolkit === | ||
| - | As a continuation of [https://2010.igem.org/Team:Washington/Tools_Used/Next-Gen_Cloning 2010 UW IGEM project], this year we | + | As a continuation of the [https://2010.igem.org/Team:Washington/Tools_Used/Next-Gen_Cloning 2010 UW IGEM project], this year we developed and submitted several plasmid backbones that are Gibson cloning method friendly-- aka pGA vectors. It is called the [https://2011.igem.org/Team:Washington/Magnetosomes/GibsonVectors Gibson Assembly Toolkit] |
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'''What’s in the Magnetosome Toolkit?''' | '''What’s in the Magnetosome Toolkit?''' | ||
| - | *A set of the 18 essential genes for the various steps | + | *A set of the 18 essential genes for the various steps of magnetosome formation. |
*Our favorite genes in pGA vectors | *Our favorite genes in pGA vectors | ||
*A table compiling individual gene functions from our literature search | *A table compiling individual gene functions from our literature search | ||
Revision as of 23:24, 22 September 2011
iGEM Toolkits
As with the expansion of the iGEM competition, many iGEM teams have started to investigate the possibility of working with large-scale genomes. Large-scale gene manipulation often requires the use of tools which allow multiple gene inserts as to bring the cloning project from single gene level to a multiple gene level. However, the current BioBrick standard vectors available through iGEM are not designed for multiple-insert cloning. Therefore, the UW iGEM team decided to research methods to improve cloning efficiency and as a result, two "toolkits" were submitted to the registry.
Gibson Assembly Toolkit
As a continuation of the 2010 UW IGEM project, this year we developed and submitted several plasmid backbones that are Gibson cloning method friendly-- aka pGA vectors. It is called the Gibson Assembly Toolkit
What's in the Gibson Assembly Toolkit?
- Five plasmid backbones
- pGA1A3, pGA1C3, pGA3K3, pGA4A5, pGA4C5
Magnetosome Toolkit
In addition, we were also ambitious about assembling a large gene-construct of over 16 kb. Therefore, utilizing our pGA vectors and Gibson cloning methods, the Magnetosome Toolkit was developed with the goal to build magnetic E.Coli; a novel characteristic expressed solely by magnetotactic bacteria, such as Magnetospirillum magneticum strain AMB-1
What’s in the Magnetosome Toolkit?
- A set of the 18 essential genes for the various steps of magnetosome formation.
- Our favorite genes in pGA vectors
- A table compiling individual gene functions from our literature search
